Thioesterases and cells for production of tailored oils

ABSTRACT

The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No. 13/837,996, filed on Mar. 15, 2013, issued as U.S. Pat. No. 9,290,749 on Mar. 22, 2016, which is hereby incorporated herein by reference in its entirety for all purposes.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jul. 3, 2013, is named SOLAP019US_SL.txt and is 318,250 bytes in size.

BACKGROUND

Certain organisms including plants and some microalgae use a type II fatty acid biosynthetic pathway, characterized by the use of discrete, monofunctional enzymes for fatty acid synthesis. In contrast, mammals and fungi use a single, large, multifunctional protein.

Type II fatty acid biosynthesis typically involves extension of a growing acyl-ACP (acyl-carrier protein) chain by two carbon units followed by cleavage by an acyl-ACP thioesterase. In plants, two main classes of acyl-ACP thioesterases have been identified: (i) those encoded by genes of the FatA class, which tend to hydrolyze oleoyl-ACP into oleate (an 18:1 fatty acid) and ACP, and (ii) those encoded by genes of the FatB class, which liberate C8-C16 fatty acids from corresponding acyl-ACP molecules.

Different FatB genes from various plants have specificities for different acyl chain lengths. As a result, different gene products will produce different fatty acid profiles in plant seeds. See, U.S. Pat. Nos. 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482; 5,298,421; 5,667,997; and U.S. Pat. Nos. 5,344,771; 5,304,481. Recently, FatB genes have been cloned into oleaginous microalgae to produce triglycerides with altered fatty acid profiles. See, WO2010/063032, WO2011,150411, and WO2012/106560.

SUMMARY

In an embodiment of the invention, there is a nucleic acid having at least 80% sequence identity to any of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76 or any equivalent sequences by virtue of the degeneracy of the genetic code.

In another embodiment of the invention, there is a nucleic acid sequence encoding a protein having at least 80% sequence identity to any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77, or a fragment thereof having acyl-ACP thioesterase activity. The protein can have acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell comprising that sequence.

In a further embodiment of the invention there is a method of producing a recombinant host cell that produces an altered fatty acid profile, the method comprising transforming the cell with any of the nucleic acids mentioned above. The host cell can be a plant cell, a microbial cell, or a microalgal cell. Another embodiment of the invention includes a host cell produced by this method.

In an embodiment, there is a method for producing an oil or oil-derived product, the method comprising cultivating the host cell and extracting the oil, optionally wherein the cultivation is heterotrophic growth on sugar. Optionally, a fatty acid, fuel, chemical, or other oil-derived product can be produced from the oil. Optionally, the oil can have a fatty acid profile comprising at least 20% C8, C10, C12, C14 or C16 fatty acids. Optionally, the oil is produced by a microalgae and can lack C24-alpha sterols.

DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS OF THE INVENTION Definitions

As used with respect to nucleic acids, the term “isolated” refers to a nucleic acid that is free of at least one other component that is typically present with the naturally occurring nucleic acid. Thus, a naturally occurring nucleic acid is isolated if it has been purified away from at least one other component that occurs naturally with the nucleic acid.

A “natural oil” or “natural fat” shall mean a predominantly triglyceride oil obtained from an organism, where the oil has not undergone blending with another natural or synthetic oil, or fractionation so as to substantially alter the fatty acid profile of the triglyceride. In connection with an oil comprising triglycerides of a particular regiospecificity, the natural oil or natural fat has not been subjected to interesterification or other synthetic process to obtain that regiospecific triglyceride profile, rather the regiospecificity is produced naturally, by a cell or population of cells. In connection with a natural oil or natural fat, and as used generally throughout the present disclosure, the terms oil and fat are used interchangeably, except where otherwise noted. Thus, an “oil” or a “fat” can be liquid, solid, or partially solid at room temperature, depending on the makeup of the substance and other conditions. Here, the term “fractionation” means removing material from the oil in a way that changes its fatty acid profile relative to the profile produced by the organism, however accomplished. The terms “natural oil” and “natural fat” encompass such oils obtained from an organism, where the oil has undergone minimal processing, including refining, bleaching and/or degumming, that does not substantially change its triglyceride profile. A natural oil can also be a “noninteresterified natural oil”, which means that the natural oil has not undergone a process in which fatty acids have been redistributed in their acyl linkages to glycerol and remain essentially in the same configuration as when recovered from the organism.

“Exogenous gene” shall mean a nucleic acid that codes for the expression of an RNA and/or protein that has been introduced into a cell (e.g. by transformation/transfection), and is also referred to as a “transgene”. A cell comprising an exogenous gene may be referred to as a recombinant cell, into which additional exogenous gene(s) may be introduced. The exogenous gene may be from a different species (and so heterologous), or from the same species (and so homologous), relative to the cell being transformed. Thus, an exogenous gene can include a homologous gene that occupies a different location in the genome of the cell or is under different control, relative to the endogenous copy of the gene. An exogenous gene may be present in more than one copy in the cell. An exogenous gene may be maintained in a cell as an insertion into the genome (nuclear or plastid) or as an episomal molecule.

“Fatty acids” shall mean free fatty acids, fatty acid salts, or fatty acyl moieties in a glycerolipid. It will be understood that fatty acyl groups of glycerolipids can be described in terms of the carboxylic acid or anion of a carboxylic acid that is produced when the triglyceride is hydrolyzed or saponified.

“Microalgae” are microbial organisms that contain a chloroplast or other plastid, and optionally that are capable of performing photosynthesis, or a prokaryotic microbial organism capable of performing photosynthesis. Microalgae include obligate photoautotrophs, which cannot metabolize a fixed carbon source as energy, as well as heterotrophs, which can live solely off of a fixed carbon source. Microalgae include unicellular organisms that separate from sister cells shortly after cell division, such as Chlamydomonas, as well as microbes such as, for example, Volvox, which is a simple multicellular photosynthetic microbe of two distinct cell types. Microalgae include cells such as Chlorella, Dunaliella, and Prototheca. Microalgae also include other microbial photosynthetic organisms that exhibit cell-cell adhesion, such as Agmenellum, Anabaena, and Pyrobotrys. Microalgae also include obligate heterotrophic microorganisms that have lost the ability to perform photosynthesis, such as certain dinoflagellate algae species and species of the genus Prototheca.

An “oleaginous” cell is a cell capable of producing at least 20% lipid by dry cell weight, naturally or through recombinant or classical strain improvement. An “oleaginous microbe” or “oleaginous microorganism” is a microbe, including a microalga that is oleaginous.

In connection with a natural oil, a “profile” is the distribution of particular species or triglycerides or fatty acyl groups within the oil. A “fatty acid profile” is the distribution of fatty acyl groups in the triglycerides of the oil without reference to attachment to a glycerol backbone. Fatty acid profiles are typically determined by conversion to a fatty acid methyl ester (FAME), followed by gas chromatography (GC) analysis with flame ionization detection (FID). The fatty acid profile can be expressed as one or more percent of a fatty acid in the total fatty acid signal determined from the area under the curve for that fatty acid. FAME-GC-FID measurement approximate weight percentages of the fatty acids.

“Recombinant” is a cell, nucleic acid, protein or vector that has been modified due to the introduction of an exogenous nucleic acid or the alteration of a native nucleic acid. Thus, e.g., recombinant cells can express genes that are not found within the native (non-recombinant) form of the cell or express native genes differently than those genes are expressed by a non-recombinant cell. Recombinant cells can, without limitation, include recombinant nucleic acids that encode a gene product or suppression elements such as mutations, knockouts, antisense, interfering RNA (RNAi) or dsRNA that reduce the levels of active gene product in a cell. A “recombinant nucleic acid” is a nucleic acid originally formed in vitro, in general, by the manipulation of nucleic acid, e.g., using polymerases, ligases, exonucleases, and endonucleases, using chemical synthesis, or otherwise is in a form not normally found in nature. Recombinant nucleic acids may be produced, for example, to place two or more nucleic acids in operable linkage. Thus, an isolated nucleic acid or an expression vector formed in vitro by ligating DNA molecules that are not normally joined in nature, are both considered recombinant for the purposes of this invention. Once a recombinant nucleic acid is made and introduced into a host cell or organism, it may replicate using the in vivo cellular machinery of the host cell; however, such nucleic acids, once produced recombinantly, although subsequently replicated intracellularly, are still considered recombinant for purposes of this invention. Similarly, a “recombinant protein” is a protein made using recombinant techniques, i.e., through the expression of a recombinant nucleic acid.

Thioesterase Sequences

Additional FatB genes encoding thioesterases with varying substrate preferences have been identified from plant seeds. These genes or functional subsequences thereof can be used to engineer organisms to produce fatty acids having a chain-length distribution (fatty acid profile) that is altered from the wild type organism. Specifically, recombinant cells express one or more of the exogenous FatB genes. The fatty acids can be further converted to triglycerides, fatty aldehydes, fatty alcohols and other oleochemicals either synthetically or biosynthetically. In specific embodiments, triglycerides are produced by a host cell expressing the novel FatB gene. A triglyceride-containing natural oil can be recovered from the host cell. The natural oil can be refined, degummed, bleached and/or deodorized. The oil, in its natural or processed form, can be used for foods, chemicals, fuels, cosmetics, plastics, and other uses.

The genes can be used in a variety of genetic constructs including plasmids or other vectors for expression or recombination in a host cell. The genes can be codon optimized for expression in a target host cell. The proteins produced by the genes can be used in vivo or in purified form.

The gene sequences disclosed can also be used to prepare antisense, or inhibitory RNA (e.g., RNAi or hairpin RNA) to inhibit complementary genes in a plant or other organism.

FatB genes found to be useful in producing desired fatty acid profiles in a cell are summarized below in Table 1. Nucleic acids or proteins having the sequence of SEQ ID NOS: 1-78 can be used to alter the fatty acid profile of a recombinant cell. Variant nucleic acids can also be used; e.g, variants having at least 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, or 78. Codon optimization of the genes for a variety of host organisms is contemplated, as is the use of gene fragments. Preferred codons for Prototheca strains and for Chlorella protothecoides are shown below in Tables 2 and 3, respectively. In some embodiments, the first and/or second most preferred Prototheca codons are employed for codon optimization.

In embodiments of the invention, there is protein or a nucleic acid encoding a protein having any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77. In an embodiment, there is protein or a nucleic acid encoding a protein having at least 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% sequence identity with any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77. In certain embodiments, the invention encompasses a fragment any of the above-described proteins or nucleic acids (including fragments of protein or nucleic acid variants), wherein the protein fragment has acyl-ACP thioesterase activity or the nucleic acid fragment encodes such a protein fragment. In other embodiments, the fragment includes a domain of an acyl-ACP thioesterase that mediates a particular function, e.g., a specificity-determining domain. Illustrative fragments can be produced by C-terminal and/or N-terminal truncations and include at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the full-length sequences disclosed herein.

The term “percent sequence identity,” in the context of two or more amino acid or nucleic acid sequences, refers to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence, as measured using a sequence comparison algorithm or by visual inspection. For sequence comparison to determine percent nucleotide or amino acid identity, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. Optimal alignment of sequences for comparison can be conducted using the NCBI BLAST software (ncbi.nlm.nih.gov/BLAST/) set to default parameters. For example, to compare two nucleic acid sequences, one may use blastn with the “BLAST 2 Sequences” tool Version 2.0.12 (Apr. 21, 2000) set at the following default parameters: Matrix: BLOSUM62; Reward for match: 1; Penalty for mismatch: −2; Open Gap: 5 and Extension Gap: 2 penalties; Gap x drop-off: 50; Expect: 10; Word Size: 11; Filter: on. For a pairwise comparison of two amino acid sequences, one may use the “BLAST 2 Sequences” tool Version 2.0.12 (Apr. 21, 2000) with blastp set, for example, at the following default parameters: Matrix: BLOSUM62; Open Gap: 11 and Extension Gap: 1 penalties; Gap x drop-off 50; Expect: 10; Word Size: 3; Filter: on.

In certain embodiments, percent sequence identity for variants of the nucleic acids or proteins discussed above can be calculated by using the full-length nucleic acid sequence (e.g., one of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, or 78) or full-length amino acid sequence (e.g., one of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77) as the reference sequence and comparing the full-length test sequence to this reference sequence. In some embodiments relating to fragments, percent sequence identity for variants of nucleic acid or protein fragments can be calculated over the entire length of the fragment.

The nucleic acids can be in isolated form, or part of a vector or other construct, chromosome or host cell. It has been found that is many cases the full length gene (and protein) is not needed; for example, deletion of some or all of the N-terminal hydrophobic domain (typically an 18 amino acid domain starting with LPDW (SEQ ID NO: 115)) yields a still-functional gene. In addition, fusions of the specificity determining regions of the genes in Table 1 with catalytic domains of other acyl-ACP thioesterases can yield functional genes. Thus, in certain embodiments, the invention encompasses functional fragments (e.g., specificity determining regions) of the disclosed nucleic acid or amino acids fused to heterologous acyl-ACP thioesterase nucleic acid or amino acid sequences, respectively.

TABLE 1 FatB genes according to embodiments of the present invention Native CDS Prototheca nucloetide moriformis Sequence Amino Acid sequence (not codon- Variant (relative Sequence of codon- optimized to dominant CDS (no optimized, no nucleotide transcript additional additional sequence Species Gene Name idenitified) tags) cloning sites) of CDS Cinnamomum CcFATB1b M25L, M322R, SEQ ID NO: 1 SEQ ID NO: 2 SEQ ID camphora ΔT367-D368 NO: 3 Cinnamomum CcFATB4 “wild-type” SEQ ID NO: 4 SEQ ID NO: 5 SEQ ID camphora NO: 6 Cinnamomum CcFATB3 “wild-type” SEQ ID NO: 7 SEQ ID NO: 8 SEQ ID camphora NO: 9 Cuphea ChsFATB1 “wild-type” SEQ ID NO: SEQ ID NO: 11 SEQ ID hyssopifolia 10 NO: 12 Cuphea ChsFATB2 “wild-type” SEQ ID NO: SEQ ID NO: 14 SEQ ID hyssopifolia 13 NO: 15 Cuphea ChsFATB2b +a.a.248-259 SEQ ID NO: SEQ ID NO: 17 SEQ ID hyssopifolia 16 NO: 18 Cuphea ChsFATB3 “wild-type” SEQ ID NO: SEQ ID NO: 20 SEQ ID hyssopifolia 19 NO: 21 Cuphea ChsFATB3b V204I, C239F, SEQ ID NO: SEQ ID NO: 23 SEQ ID hyssopifolia E243D, M251V 22 NO: 24 Cuphea CuPSR23FATB3 “wild-type” SEQ ID NO: SEQ ID NO: 26 SEQ ID PSR23 25 NO: 27 Cuphea CwFATB3 “wild-type” SEQ ID NO: SEQ ID NO: 29 SEQ ID wrightii 28 NO: 30 Cuphea CwFATB4a “wild-type” SEQ ID NO: SEQ ID NO: 32 SEQ ID wrightii 31 NO: 33 Cuphea CwFATB4b “wild-type” SEQ ID NO: SEQ ID NO: 35 SEQ ID wrightii 34 NO: 36 Cuphea CwFATB5 “wild-type” SEQ ID NO: SEQ ID NO: 38 SEQ ID wrightii 37 NO: 39 Cuphea ChtFATB1a “wild-type” SEQ ID NO: SEQ ID NO: 41 SEQ ID heterophylla 40 NO: 42 Cuphea ChtFATB1b P16S, T20P, G94S, SEQ ID NO: SEQ ID NO: 44 SEQ ID heterophylla G105W, S293F, 43 NO: 45 L305F Cuphea ChtFATB2b “wild-type” SEQ ID NO: SEQ ID NO: 47 SEQ ID heterophylla 46 NO: 48 Cuphea ChtFATB2a S17P, P21S, T28N, SEQ IDO NO: SEQ ID NO: 50 SEQ ID heterophylla L30P, S33L, 49 NO: 51 G76D, S78P, G137W Cuphea ChtFATB2c G76D, S78P SEQ ID NO: SEQ ID NO: 53 SEQ ID heterophylla 52 NO: 54 Cuphea ChtFATB2d S21P, T28N, SEQ ID NO: SEQ ID NO: 56 SEQ ID heterophylla L30P, S33L, 55 NO: 57 G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A Cuphea ChtFATB2e G76D, R97L, SEQ ID NO: SEQ ID NO: 59 SEQ ID heterophylla H124L, I132S, 58 NO: 60 G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A Cuphea ChtFATB2f R97L, H124L, SEQ ID NO: SEQ ID NO: 62 SEQ ID heterophylla I132S, G152S, 61 NO: 63 H165L, T211N Cuphea ChtFATB2g A6T, A16V, S17P, SEQ ID NO: SEQ ID NO: 65 SEQ ID heterophylla G76D, R97L, 64 NO: 66 H124L, I132S, S143I, G152S, A157T, H165L, T211N, G414A Cuphea ChtFATB3a “wild-type” SEQ ID NO: SEQ ID NO: 68 SEQ ID heterophylla 67 NO: 69 Cuphea ChtFATB3b C67G, H72Q, SEQ ID NO: SEQ ID NO: 71 SEQ ID heterophylla L28F, N179I 70 NO: 72 Cuphea CvisFATB1 published SEQ ID NO: N/A SEQ ID viscosissima 73 NO: 74 Cuphea CvisFATB2 published SEQ ID NO: N/A SEQ ID viscosissima 75 NO: 76 Cuphea CvisFATB3 published SEQ ID NO: N/A SEQ ID viscosissima 77 NO: 78

TABLE 2 Preferred codon usage in Prototheca strains Ala GCG 345 (0.36) ASN AAT   8 (0.04) GCA  66 (0.07) AAC 201 (0.96) GCT 101 (0.11) GCC 442 (0.46) Cys TGT  12 (0.10) Pro CCG 161 (0.29) TGC 105 (0.90) CCA  49 (0.09) CCT  71 (0.13) CCC 267 (0.49) Asp GAT  43 (0.12) Gln CAG 226 (0.82) GAC 316 (0.88) CAA  48 (0.18) Glu GAG 377 (0.96) Arg AGG  33 (0.06) GAA  14 (0.04) AGA  14 (0.02) CGG 102 (0.18) CGA  49 (0.08) CGT  51 (0.09) CGC 331(0.57) Phe TTT  89 (0.29) Ser AGT  16 (0.03) TTC 216 (0.71) AGC 123 (0.22) TCG 152 (0.28) TCA  31 (0.06) TCT  55 (0.10) TCC 173 (0.31) Gly GGG  92 (0.12) Thr ACG 184 (0.38) GGA  56 (0.07) ACA  24 (0.05) GGT  76 (0.10) ACT  21 (0.05) GGC 559 (0.71) ACC 249 (0.52) His CAT  42 (0.21) Val GTG 308 (0.50) CAC 154 (0.79) GTA   9 (0.01) GTT  35 (0.06) GTC 262 (0.43) Ile ATA   4 (0.01) Trp TGG 107 (1.00) ATT  30 (0.08) ATC 338 (0.91) Lys AAG 284 (0.98) Tyr TAT  10 (0.05) AAA   7 (0.02) TAC 180 (0.95) Leu TTG  26 (0.04) Stop TGA/TAG/TAA TTA   3 (0.00) CTG 447 (0.61) CTA  20 (0.03) CTT  45 (0.06) CTC 190 (0.26) Met ATG 191 (1.00)

TABLE 3 Preferred codon usage in Chlorella protothecoides TTC (Phe) TAC (Tyr) TGC (Cys) TGA (Stop) TGG (Trp) CCC (Pro) CAC (His) CGC (Arg) CTG (Leu) CAG (Gln) ATC (Ile) ACC (Thr) GAC (Asp) TCC (Ser) ATG (Met) AAG (Lys) GCC (Ala) AAC (Asn) GGC (Gly) GTG (Val) GAG (Glu)

Host Cells

The host cell can be a single cell or part of a multicellular organism such as a plant. Methods for expressing Fatb genes in a plant are given in U.S. Pat. Nos. 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482; 5,298,421; 5,667,997; and U.S. Pat. Nos. 5,344,771; 5,304,481, or can be accomplished using other techniques generally known in plant biotechnology. Engineering of oleaginous microbes including Chlorophyta is disclosed in WO2010/063032, WO2011,150411, and WO2012/106560 and in the examples below.

Examples of oleaginous host cells include plant cells and microbial cells having a type II fatty acid biosynthetic pathway, including plastidic oleaginous cells such as those of oleaginous algae. Specific examples of microalgal cells include heterotrophic or obligate heterotrophic microalgae of the phylum Chlorophtya, the class Trebouxiophytae, the order Chlorellales, or the family Chlorellacae. Examples of oleaginous microalgae are provided in Published PCT Patent Applications WO2008/151149, WO2010/06032, WO2011/150410, and WO2011/150411, including species of Chlorella and Prototheca, a genus comprising obligate heterotrophs. The oleaginous cells can be, for example, capable of producing 25, 30, 40, 50, 60, 70, 80, 85, or about 90% oil by cell weight, ±5%. Optionally, the oils produced can be low in DHA or EPA fatty acids. For example, the oils can comprise less than 5%, 2%, or 1% DI-IA and/or EPA. The above-mentioned publications also disclose methods for cultivating such cells and extracting oil, especially from microalgal cells; such methods are applicable to the cells disclosed herein and incorporated by reference for these teachings. When microalgal cells are used they can be cultivated autotrophically (unless an obligate heterotroph) or in the dark using a sugar (e.g., glucose, fructose and/or sucrose). In any of the embodiments described herein, the cells can be heterotrophic cells comprising an exogenous invertase gene so as to allow the cells to produce oil from a sucrose feedstock. Alternately, or in addition, the cells can metabolize xylose from cellulosic feedstocks. For example, the cells can be genetically engineered to express one or more xylose metabolism genes such as those encoding an active xylose transporter, a xylulose-5-phosphate transporter, a xylose isomerase, a xylulokinase, a xylitol dehydrogenase and a xylose reductase. See WO2012/154626, “GENETICALLY ENGINEERED MICROORGANISMS THAT METABOLIZE XYLOSE”, published Nov. 15, 2012.

Oils and Related Products

The oleaginous cells express one or more exogenous genes encoding fatty acid biosynthesis enzymes. As a result, some embodiments feature natural oils that were not obtainable from a non-plant or non-seed oil, or not obtainable at all.

The oleaginous cells produce a storage oil, which is primarily triacylglyceride and may be stored in storage bodies of the cell. A raw oil may be obtained from the cells by disrupting the cells and isolating the oil. WO2008/151149, WO2010/06032, WO2011/150410, and WO2011/1504 disclose heterotrophic cultivation and oil isolation techniques. For example, oil may be obtained by cultivating, drying and pressing the cells. The oils produced may be refined, bleached and deodorized (RBD) as known in the art or as described in WO2010/120939. The raw or RBD oils may be used in a variety of food, chemical, and industrial products or processes. After recovery of the oil, a valuable residual biomass remains. Uses for the residual biomass include the production of paper, plastics, absorbents, adsorbents, as animal feed, for human nutrition, or for fertilizer.

Where a fatty acid profile of a triglyceride (also referred to as a “triacylglyceride” or “TAG”) cell oil is given here, it will be understood that this refers to a nonfractionated sample of the storage oil extracted from the cell analyzed under conditions in which phospholipids have been removed or with an analysis method that is substantially insensitive to the fatty acids of the phospholipids (e.g. using chromatography and mass spectrometry). The oil may be subjected to an RBD process to remove phospholipids, free fatty acids and odors yet have only minor or negligible changes to the fatty acid profile of the triglycerides in the oil. Because the cells are oleaginous, in some cases the storage oil will constitute the bulk of all the TAGs in the cell.

The stable carbon isotope value δ13C is an expression of the ratio of 13C/12C relative to a standard (e.g. PDB, carbonite of fossil skeleton of Belemnite americana from Peedee formation of South Carolina). The stable carbon isotope value δ13C (0/00) of the oils can be related to the δ13C value of the feedstock used. In some embodiments, the oils are derived from oleaginous organisms heterotrophically grown on sugar derived from a C4 plant such as corn or sugarcane. In some embodiments the δ13C (0/00) of the oil is from −10 to −17 0/00 or from −13 to −16 0/00.

The oils produced according to the above methods in some cases are made using a microalgal host cell. As described above, the microalga can be, without limitation, fall in the classification of Chlorophyta, Trebouxiophyceae, Chlorellales, Chlorellaceae, or Chlorophyceae. It has been found that microalgae of Trebouxiophyceae can be distinguished from vegetable oils based on their sterol profiles. Oil produced by Chlorella protothecoides was found to produce sterols that appeared to be brassicasterol, ergosterol, campesterol, stigmasterol, and β-sitosterol, when detected by GC-MS. However, it is believed that all sterols produced by Chlorella have C24β stereochemistry. Thus, it is believed that the molecules detected as campesterol, stigmasterol, and β-sitosterol, are actually 22,23-dihydrobrassicasterol, proferasterol and clionasterol, respectively. Thus, the oils produced by the microalgae described above can be distinguished from plant oils by the presence of sterols with C24β stereochemistry and the absence of C24α stereochemistry in the sterols present. For example, the oils produced may contain 22,23-dihydrobrassicasterol while lacking campesterol; contain clionasterol, while lacking in β-sitosterol, and/or contain poriferasterol while lacking stigmasterol. Alternately, or in addition, the oils may contain significant amounts of Δ⁷-poriferasterol.

In embodiments of the present invention, oleaginous cells expressing one or more of the genes of Table 1 can produce an oil with at least 20% of C8, C10, C12, C14 or C16 fatty acids. In a specific embodiment, the level of myristate (C14:0) in the oil is greater than 30%.

Thus, in embodiments of the invention, there is a process for producing an oil, triglyceride, fatty acid, or derivative of any of these, comprising transforming a cell with any of the nucleic acids discussed herein. In another embodiment, the transformed cell is cultivated to produce an oil and, optionally, the oil is extracted. Oil extracted in this way can be used to produce food, oleochemicals or other products.

The oils discussed above alone or in combination are useful in the production of foods, fuels and chemicals (including plastics, foams, films, etc). The oils, triglycerides, fatty acids from the oils may be subjected to C—H activation, hydroamino methylation, methoxy-carbonation, ozonolysis, enzymatic transformations, epoxidation, methylation, dimerization, thiolation, metathesis, hydro-alkylation, lactonization, or other chemical processes.

After extracting the oil, a residual biomass may be left, which may have use as a fuel, as an animal feed, or as an ingredient in paper, plastic, or other product. For example, residual biomass from heterotrophic algae can be used in such products.

The described embodiments of the invention are intended to be merely exemplary and numerous variations and modifications will be apparent to those skilled in the art. All such variations and modifications are intended to be within the scope of the present invention. For example, the various triglyceride oils can be tailored in for a mixture of midchain and long chain fatty acids in order to adjust parameters such as polarity, solvency, and foam-height of the oils or chemicals made from the oils.

Example 1

Sequences of novel plant acyl-ACP thioesterases involved in seed-specific midchain (C8-C16) fatty acid biosynthesis in higher plants were isolated. Seed-specific lipid production genes were isolated through direct interrogation of RNA pools accumulating in oilseeds. Based on phylogenetic analysis, novel enzymes can be classified as members of FatB family of acyl-ACP thioesterases.

Seeds of oleaginous plants were obtained from local grocery stores or requested through USDA ARS National Plant Germplasm System (NPGS) from North Central Regional Plant Introduction Station (NCRIS) or USDA ARS North Central Soil Conservation Research Laboratory (Morris, Mich.). Dry seeds were homogenized in liquid nitrogen to powder, resuspended in cold extraction buffer containing 6-8M Urea and 3M LiCl and left on ice for a few hours to overnight at 4° C. The seed homogenate was passed through NucleoSpin Filters (Macherey-Nagel) by centrifugation at 20,000 g for 20 minutes in the refrigerated microcentrifuge (4° C.). The resulting RNA pellets were resuspended in the buffer containing 20 mM Tris HCl, pH7.5, 0.5% SDS, 100 mM NaCl, 25 mM EDTA, 2% PVPP) and RNA was subsequently extracted once with Phenol-Chloroform-Isoamyl Alcohol (25:24:1, v/v) and once with chloroform. RNA was finally precipitated with isopropyl alcohol (0.7 Vol.) in the presence of 150 mM of Na Acetate, pH5.2, washed with 80% ethanol by centrifugation, and dried. RNA samples were treated with Turbo DNAse (Lifetech) and purified further using RNeasy kits (Qiagen) following manufacturers' protocols. The resulting purified RNA samples were converted to pair-end cDNA libraries and subjected to next-generation sequencing (2×100 bp) using Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages. Putative thioesterase-containg cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs have been further verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes.

To interrogate evolutionary and functional relationship between novel acyl-ACP thioesterases and the members of two existing thioesterase classes (FatA and FatB), we performed a phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) amino acid thioesterase sequences. Novel proteins appear to group with known acyl-ACP FatB thioesterases involved in biosynthesis of C8-C16 fatty acids. Moreover, novel thioesterases appear to cluster into 3 predominant out-groups suggesting distinct functional similarity and evolutionary relatedness among members of each cluster.

The amino acid sequences of the FatB genes follow are shown in Table 4.

TABLE 4 Amino acid sequences of FatB genes CuPSR23 FATB3 (SEQ ID NO: 25): MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAH PKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLD RKSKRPDMLVDSVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCK SLGLLNDGFGRTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASD WLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLH KFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTST AKTSNGNSVS CuPSR23 FATB3b (SEQ ID NO: 79): MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAH PKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLD RKSKRPDMLVDSVGLKSIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKS LGLLNDGFGRTPGMCKNDLIWVLTKMQIIVIVNRYPTWGDTVEINTWFSQSGKIGMASD WLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLH KFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTST AKTSNGNSAS CwFATB3 (SEQ ID NO: 28): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRR ECGRESVVESVTSMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST CwFATB3a (SEQ ID NO: 28): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRR ECGRESVVESVTSMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST CwFATB3b (SEQ ID NO: 80): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVE GKVW CwFATB3c (SEQ ID NO: 81): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVATEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVE GKVW CwFATB4a (SEQ ID NO: 31): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSE ESSPGDFF CwFATB4a.1 (SEQ ID NO: 82): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINWVVPSE ESSPGDFF CwFATB4a.2 (SEQ ID NO: 83): MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSE ESSPGDFF CwFATB4a.3 (SEQ ID NO: 84): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYVSRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSE ESSPGDFF CwFATB4b (SEQ ID NO: 34): MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSSDGFGRTPAMSKRDLIWVVAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGEGDGSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPS EESSPGGDFF CwFATB4b.1 (SEQ ID NO: 85): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGSFKTQEDAPSAPPPRTFrNQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVIVINHLQETALNHVKI AGLSSDGFGRTPAMSKRDLIWVVAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGEGDGSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPS EESSPGGDFF CwFATB5 (SEQ ID NO: 37): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVV RLIF CwFATB5a (SEQ ID NO: 86): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGFFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVV RLIF CwFATB5b (SEQ ID NO: 87): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLWLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRV VRLIF CwFATB5c (SEQ ID NO: 88): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPIWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGMGWQPFRVV RLIF CwFATB5.1 (SEQ ID NO: 89): MVAAAASSAFFSVPTPGTSPKPGKFRNWPSSLSVPFKPETNHNGGFHIKANASAH PKANGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGM LVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGE ILIRATSVWAMMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIR DGLTPRWNDLDVNQHVNNVKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLES VTAMDPAKEGDRSVYQHLLRLEDGADITIGRTEWRPKNAGANEAISSGKTSNGNSAS CwFATB5.1a (SEQ ID NO: 90): MVAAAASSAFFSVPTPGTSPKPGKFRNWPLSLSVPFKPETNHNGGFHIKANASAH PKANGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGM LVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTAKETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGE ILIRATSVWAMMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDS1R DGLTPRWNDLDVNQHVNNVKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLES VTAMDPAKEGDRSVYQHLLRLEDGADITIGRTEWRPKNAGANEAISSGKTSNGNSAS CcFATB2b (SEQ ID NO: 91): MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKD TEGLKGCSTLQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAAR NHAESLGLLGDGFGETLEMSKRDLIWVVRRTHVAVERYPAWGDTVEVEAWVGASGNT GMRRDFLVRDCKTGHILTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEI KKLQKLNDSTADYIQGGWTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLE YRRECIRGNKLQSLTTVCGGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISER FPQQEPHK CcFATB3 (SEQ ID NO: 7): MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKAN AHASPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNL DWKPRRPDMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNH VRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNG MRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDN RKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTL EYRRECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKS ANNSRSILEMPAESL CcFATB3b (SEQ ID NO: 92): MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKLASSSGLQVKAN AHASPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNL DWKPRRPDMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNH VRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNG MRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDN RKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTL EYRRECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKS ANNSRSILEMPAESL CcFATB3c (SEQ ID NO: 93): MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKAN AHASPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNL DWKPRRPDMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNH VRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNG MRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDN RKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNAKYIGWILESAPGSILESHELSCMTL EYRRECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKS ANNSRSILEMPAESL ChtFATB1a (SEQ ID NO: 40): MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDLDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB1a.1 (SEQ ID NO: 94): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRHSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLIGDC RTGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTG DSIRKGLTPRWNDLDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDS VLESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGALSTGKTSNGN SVS ChtFATB1a.2 (SEQ ID NO: 95): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSNLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB1a.3 (SEQ ID NO: 96): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSINQHLLRLEDGADITIGRTEWRPKNAGVNGAISTGKTSNENSVS ChtFATB1a.4 (SEQ ID NO: 97): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB1b (SEQ ID NO: 43): MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPESSHNGGFQVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD FIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB2b (SEQ ID NO: 46): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLD RKSKKPDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRE CGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGK TSNGNSVS ChtFATB2a (SEQ ID NO: 49): MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANAS AHPKANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTML DRKSKKPDMHVDWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHC KSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGR NWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPLIEDNDRKLH KFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRR ECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTG KTSNGNSVS ChtFATB2c (SEQ ID NO: 52): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTML DRKSKKPDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETININHLQDTSLNHC KSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGR NWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRR ECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTG KTSNGNSVS ChtFATB2d (SEQ ID NO: 55): MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANAS AHPKANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTML DRKSKRPDMLVDLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHC KSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGR NWLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRR ECGRESVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTG KTSNGNSVS ChtFATB2e (SEQ ID NO: 58): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCK SMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRE CGRDSVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGK TSNGNSVS ChtFATB2f (SEQ ID NO: 61): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCK SMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRE CGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGK TSNGNSVS ChtFATB2g (SEQ ID NO: 64): MVVAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKS MGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRE CGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGK TSNANSVS ChtFATB2h (SEQ ID NO: 98): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCK SMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESIPTEVLETQELCSLTLEYRREC GRESVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKT SNGNSVS ChtFATB3a (SEQ ID NO: 67): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChtFATB3b (SEQ ID NO: 70): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLIEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRR DWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKL PKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGE TSPGNS ChtFATB3c (SEQ ID NO: 99): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDRK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSEKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGAIAFG ETSPGDS ChtFATB3d (SEQ ID NO: 100): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIKTVMNHLQETALNHVK SAGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGM RRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDW KLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLE YRRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIAS GETSPGNS ChtFATB3e (SEQ ID NO: 101): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSGSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChtFATB3f (SEQ ID NO: 102): MVATAASSAFFPVPSPDTSSRLGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMPVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSEKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChtFATB3g (SEQ ID NO: 103): MVATAASSAFFPVPSPDTSSRAGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGS SVS LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHEVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChsFATB1 (SEQ ID NO: 10): MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAAR NGALPLLASICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAI NHVQSAGYFGDSFGATPEMTKRNLIWVITKMQVLVDRYPAWGDVVQVDTWTCSSGKN SMQRDWFVRDLKTGDIITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDD NRKLPKLDGSSADYVLSGLTPRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAIT VEYRRECGKNSVLQSLTNVSGDGITCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRG APIQAEKVYNNK ChsFATB2 (SEQ ID NO: 13): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDD RKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTL EYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIA SGETSPGDSS ChsFatB2b (SEQ ID NO: 16): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSKSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVRHEIEPH FVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPE VLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEW RPKTAGINGPIASGETSPGDSS ChsFatB2c (SEQ ID NO: 104): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDD RKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTL EYRRECGRESVLESLTAVDPSGKGSGSQFQHLMRLEDGGEIVKGRTEWRPKTAGINGPI ASGETSPGDSS ChsFatB2d (SEQ ID NO: 105): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDD RKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTL EYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIA SGETSPGDSS Chs FATB3 (SEQ ID NO: 19): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFatb3b (SEQ ID NO: 22): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVINAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRR ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTG KTSNGNSIS ChsFatB3c (SEQ ID NO: 106): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR QECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNS IS ChsFATB3d (SEQ ID NO: 107): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRSDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3e (SEQ ID NO: 108): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRSDMLMDPFGVDRVVQDGVVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3f (SEQ ID NO: 109): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG, RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3g (SEQ ID NO: 110): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQ ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS ChsFATB3h (SEQ ID NO: 111): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRSDMLMDPFGVDRVVQDGVVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQ ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS ChsFATB3i (SEQ ID NO: 112): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRICSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGGDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3j (SEQ ID NO: 113): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQ ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS

Example 2

In the example below, we detail the effect of expressing plant oilseed transcriptome-derived, heterologous thioesterases in the UTEX1435 (web.biosci.utexas.edu/utex/) strain, Strain A.

As in Example 1, RNA was extracted from dried plant seeds and submitted for paired-end sequencing using the Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages and putative thioesterase-containing cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs were verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes and to identify sequence variants arising from expression of different gene alleles or diversity of sequences within a population of seeds. The resulting amino acid sequences were subjected to phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) FatB sequences. The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty acids, were pursued.

Construction of Transforming Vectors Expressing Acyl-ACP FatB Thioesterases

27 putative acyl-ACP FatB thioesterases from the species Cinnamomum camphora, Cuphea hyssopifolia, Cuphea PSR23, Cuphea wrightii, Cuphea heterophylla, and Cuphea viscosissima were synthesized in a codon-optimized form to reflect Prototheca moriformis (UTEX 1435) codon usage. Of the 27 genes synthesized, 24 were identified by our transcriptome sequencing efforts and the 3 genes from Cuphea viscosissima, were from published sequences in GenBank.

Transgenic strains were generated via transformation of the base strain Strain A (Prototheca moriformis, derived from UTEX 1435 by classical mutation and screening for high oil production) with a construct encoding 1 of the 27 FatB thioesterases. The construct pSZ2760 encoding Cinnamomum camphora (Cc) FATB1b is shown as an example, but identical methods were used to generate each of the remaining 26 constructs encoding the different respective thioesterases. Construct pSZ2760 can be written as 6S::CrTUB2:ScSUC2:CvNR::PmAMT3:CcFATB1b:CvNR::6S. The sequence of the transforming DNA is provided in Table 5 (pSZ2760). The relevant restriction sites in the construct from 5′-3′, BspQ1, KpnI, AscI, MfeI, EcoRI, SpeI, XhoI, SacI, BspQ1, respectively, are indicated in lowercase, bold, and underlined. BspQ1 sites delimit the 5′ and 3′ ends of the transforming DNA. Bold, lowercase sequences at the 5′ and 3′ end of the construct represent genomic DNA from UTEX 1435 that target integration to the 6S locus via homologous recombination. Proceeding in the 5′ to 3′ direction, the selection cassette has the C. reinhardtii β-tubulin promoter driving expression of the S. cerevisiae gene SUC2 (conferring the ability to grow on sucrose) and the Chlorella vulgaris Nitrate Reductase (NR) gene 3′ UTR. The promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for ScSUC2 are indicated by bold, uppercase italics, while the coding region is indicated with lowercase italics. The 3′ UTR is indicated by lowercase underlined text. The spacer region between the two cassettes is indicated by upper case text. The second cassette containing the codon optimized CcFATB1b gene (Table 5; pSZ2760) from Cinnamomum camphora is driven by the Prototheca moriformis endogenous AMT3 promoter, and has the Chlorella vulgaris Nitrate Reductase (NR) gene 3′ UTR. In this cassette, the AMT3 promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for the CcFATB1b gene are indicated in bold, uppercase italics, while the coding region is indicated by lowercase italics and the spacer region is indicated by upper case text. The 3′ UTR is indicated by lowercase underlined text. The final construct was sequenced to ensure correct reading frame and targeting sequences.

TABLE 5 pSZ2760 Transforming construct (SEQ ID NO: 114)

ctgcaggccttcctgttcctgctggccggcttcgccgccaagatcagcgcctccatgacgaacgagacgtccgaccgccccctggtgca cttcacccccaacaagggctggatgaacgaccccaacggcctgtggtacgacgagaaggacgccaagtggcacctgtacttccagt acaacccgaacgacaccgtctgggggacgcccttgttctggggccacgccacgtccgacgacctgaccaactgggaggaccagccc atcgccatcgccccgaagcgcaacgactccggcgccttctccggctccatggtggtggactacaacaacacctccggcttcttcaacga caccatcgacccgcgccagcgctgcgtggccatctggacctacaacaccccggagtccgaggagcagtacatctcctacagcctgga cggcggctacaccttcaccgagtaccagaagaaccccgtgctggccgccaactccacccagttccgcgacccgaaggtcttctggtac gagccctcccagaagtggatcatgaccgcggccaagtcccaggactacaagatcgagatctactcctccgacgacctgaagtcctgg aagctggagtccgcgttcgccaacgagggcttcctcggctaccagtacgagtgccccggcctgatcgaggtccccaccgagcaggac cccagcaagtcctactgggtgatgttcatctccatcaaccccggcgccccggccggcggctccttcaaccagtacttcgtcggcagcttc aacggcacccacttcgaggccucgacaaccagtcccgcgtggtggacttcggcaaggactactacgccctgcagaccttcttcaaca ccgacccgacctacgggagcgccctgggcatcgcgtgggcctccaactgggagtactccgccttcgtgcccaccaacccctggcgctc ctccatgtccctcgtgcgcaaguctccctcaacaccgagtaccaggccaacccggagacggagctgatcaacctgaaggccgagcc gatcctgaacatcagcaacgccggcccctggagccggttcgccaccaacaccacgttgacgaaggccaacagctacaacgtcgacc tgtccaacagcaccggcaccctggagttcgagctggtgtacgccgtcaacaccacccagacgatctccaagtccgtgttcgcggacct ctccctctggttcaagggcctggaggaccccgaggagtacctccgcatgggcttcgaggtgtccgcgtcctccttcttcctggaccgcgg gaacagcaaggtgaagttcgtgaaggagaacccctacttcaccaaccgcatgagcgtgaacaaccagcccttcaagagcgagaac gacctgtcctactacaaggtgtacggcttgctggaccagaacatcctggagctgtacttcaacgacggcgacgtcgtgtccaccaacac ctacttcatgaccaccgggaacgccctgggctccgtgaacatgacgacgggggtggacaacctgttctacatcgacaagttccaggtg

catgaaggccgtgatgctggcccgcgacggccgcggcctgaagccccgctcctccgacctgcagctgcgcgccggcaacgcccaga cctccctgaagatgatcaacggcaccaagttctcctacaccgagtccctgaagaagctgcccgactggtccatgctgttcgccgtgatc accaccatcttctccgccgccgagaagcagtggaccaacctggagtggaagcccaagcccaaccccccccagctgctggacgacca cttcggcccccacggcctggtgttccgccgcaccttcgccatccgctcctacgaggtgggccccgaccgctccacctccatcgtggccgt gatgaaccacctgcaggaggccgccctgaaccacgccaagtccgtgggcatcctgggcgacggcttcggcaccaccctggagatgt ccaagcgcgacctgatctgggtggtgaagcgcacccacgtggccgtggagcgctaccccgcctggggcgacaccgtggaggtgga gtgctgggtgggcgcctccggcaacaacggccgccgccacgacttcctggtgcgcgactgcaagaccggcgagatcctgacccgct gcacctccctgtccgtgatgatgaacacccgcacccgccgcctgtccaagatccccgaggaggtgcgcggcgagatcggccccgcct tcatcgacaacgtggccgtgaaggacgaggagatcaagaagccccagaagctgaacgactccaccgccgactacatccagggcg gcctgaccccccgctggaacgacctggacatcaaccagcacgtgaacaacatcaagtacgtggactggatcctggagaccgtgccc gactccatcttcgagtcccaccacatctcctccttcaccatcgagtaccgccgcgagtgcacccgcgactccgtgctgcagtccctgacc accgtgtccggcggctcctccgaggccggcctggtgtgcgagcacctgctgcagctggagggcggctccgaggtgctgcgcgccaag accgagtggcgccccaagctgtccttccgcggcatctccgtgatccccgccgagtcctccgtgatggactacaaggaccacgacggcg

Constructs encoding the identified heterologous FatB genes, such as CcFATB1b from pSZ2760 in Table 6, were transformed into Strain A, and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. After cultivating on sucrose under low nitrogen conditions to accumulate oil, fatty acid profiles were determined by FAME-GC. The top performer from each transformation, as judged by the ability to produce the highest level of midchain fatty acids, is shown in Table 4.

TABLE 6 Alteration of Fatty Acid Profiles in S3150 upon Expression of Heterologous FatB Thioesterases FA profile of top performer from each transformation (%; primary lipid in Strain A background) Species Gene Name SZ Plasmid Strain C8:0 C10:0 C12:0 Cinnamomum camphora CcFATB1b pSZ2760 A; T526; D1670-13 0 0 1 Cinnamomum camphora CcFATB4 pSZ2756 A; T525; D1666-31 0 1 33 Cinnamomum camphora CcFATB3 pSZ2755 A; T525; D1665-4 0 0 0 Cuphea hyssopifolia ChsFATB1 pSZ2778 A; T535; D1689-30 0 0 0 Cuphea hyssopifolia ChsFATB2 pSZ2796 A; T537; D1700-46 0 0 0 Cuphea hyssopifolia ChsFATB2b pSZ2792 A; T537; D1696-9 0 0 0 Cuphea hyssopifolia ChsFATB3 pSZ2797 A; T537; D1701-48 0 0 8 Cuphea hyssopifolia ChsFATB3b pSZ2795 A; T537; D1699-1 0 0 7 Cuphea PSR23 CuPSR23FATB3 pSZ2793 A; T537; D1697-13 0 1 0 Cuphea wrightii CwFATB3 pSZ2751 A; T525; D1661-22 0 2 17 Cuphea wrightii CwFATB4a pSZ2752 A; T525; D1662-30 0 0 0 Cuphea wrightii CwFATB4b pSZ2753 A; T525; D1663-29 0 0 0 Cuphea wrightii CwFATB5 pSZ2754 A; T525; D1664-39 0 0 0 Cuphea heterophylla ChtFATB1a pSZ2757 A; T525; D1667-19 0 0 5 Cuphea heterophylla ChtFATB1b pSZ2773 A; T535; D1685-29 0 0 2 Cuphea heterophylla ChtFATB2b pSZ2780 A; T535; D1691-8 0 0 0 Cuphea heterophylla ChtFATB2a pSZ2774 A; T537; D1702-24 0 0 0 Cuphea heterophylla ChtFATB2c pSZ2758 A; T525; D1668-22 0 0 3 Cuphea heterophylla ChtFATB2d pSZ2759 A; T526; D1669-19 0 0 4 Cuphea heterophylla ChtFATB2e pSZ2775 A; T535; D1686-23 0 1 2 Cuphea heterophylla ChtFATB2f pSZ2777 A; T535; D1688- 33 0 0 0 Cuphea heterophylla ChtFATB2g pSZ2794 A; T537; D1698-19 0 0 0 Cuphea heterophylla ChtFATB3a pSZ2776 A; T535; D1687-23 0 0 0 Cuphea heterophylla ChtFATB3b pSZ2779 A; T535; D1690- 31 0 0 0 Cuphea viscosissima CvisFATB1 pSZ2810 A; T540; D1711-30 0 1 0 Cuphea viscosissima CvisFATB2 pSZ2817 A; T547; D1718-1 0 0 0 Cuphea viscosissima CvisFATB3 pSZ2791 A; T537; D1695-1 0 0 0 A (parent strain): 0 0 0 FA profile of top performer from each transformation (%; primary lipid in Strain A background) Species C14:0 C16:0 C18:0 C18:1 C18:2 C18:3α Cinnamomum camphora 15 26 2 46 9 1 Cinnamomum camphora 4 7 2 41 10 1 Cinnamomum camphora 3 44 3 41 8 0 Cuphea hyssopifolia 2 22 4 63 8 1 Cuphea hyssopifolia 6 53 3 32 6 0 Cuphea hyssopifolia 5 26 2 56 9 1 Cuphea hyssopifolia 34 27 2 24 5 1 Cuphea hyssopifolia 29 27 1 28 6 1 Cuphea PSR23 2 24 3 61 8 1 Cuphea wrightii 9 19 2 41 8 1 Cuphea wrightii 4 48 3 36 7 1 Cuphea wrightii 5 52 3 32 6 1 Cuphea wrightii 3 27 3 57 7 1 Cuphea heterophylla 18 27 2 39 7 1 Cuphea heterophylla 7 27 3 53 8 1 Cuphea heterophylla 2 25 3 61 8 1 Cuphea heterophylla 2 27 3 59 6 0 Cuphea heterophylla 2 23 3 58 7 1 Cuphea heterophylla 4 23 3 54 9 1 Cuphea heterophylla 3 24 3 57 8 1 Cuphea heterophylla 2 28 3 57 8 1 Cuphea heterophylla 2 22 3 62 9 1 Cuphea heterophylla 5 47 4 37 7 1 Cuphea heterophylla 6 49 5 32 7 0 Cuphea viscosissima 2 24 3 60 8 0 Cuphea viscosissima 4 51 2 36 6 0 Cuphea viscosissima 8 28 2 52 8 1 2 28 3 58 7 0

Many of the acyl-ACP FatB thioesterases were found to exhibit midchain activity when expressed in Prototheca moriformis. For example, expression of CcFATB1b causes an increase in myristate levels from 2% of total fatty acids in the parent, Strain A, to ˜15% in the D1670-13 primary transformant. Other examples include CcFATB4, which exhibits an increase in laurate levels from 0% in Strain A to ˜33%, and ChsFATB3, which exhibits an increase in myristate levels to ˜34%. Although some of the acyl-ACP thioesterases did not exhibit dramatic effects on midchain levels in the current incarnation, efforts will likely develop to optimize some of these constructs.

Sequences of the Heterologous Acyl-ACP Thioesterases Identified and Transformed into P. moriformis (UTEX 1435)

A complete listing of relevant sequences for the transforming constructs, such as the deduced amino acid sequence of the encoded acyl-ACP thioesterase, the native CDS coding sequence, the Prototheca moriformis codon-optimized coding sequence, and the nature of the sequence variants examined, is provided as SEQ ID NOS: 1-78.

Sequence Listing SEQ ID NO 1: Cinnamomum camphora (Cc) FATB1b variant M25L, M322R, AT367-D368 amino acid sequence MATTSLASAFCSMKAVMLARDGRGLKPRSSDLQLRAGNAQTSLKMINGTKFSYTESLKKLPDWSMLFAVI TTIFSAAEKQWTNLEWKPKPNPPQLLDDHFGPHGLVFRRTFAIRSYEVGPDRSTSIVAVMNHLQEAALNH AKSVGILGDGFGTTLEMSKRDLIWVVKRTHVAVERYPAWGDTVEVECWVGASGNNGRRHDFLVRDCKTGE ILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPAFIDNVAVKDEEIKKPQKLNDSTADYIQGGLTPRWNDL DINQHVNNIKYVDWILETVPDSIFESHHISSFTIEYRRECTRDSVLQSLTTVSGGSSEAGLVCEHLLQLE GGSEVLRAKTEWRPKLSFRGISVIPAESSV* SEQ ID NO 2: Cinnamomum camphora (Cc) FATB1b variant M25L, M322R, 6T367-D368 coding DNA sequence TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGAAACCCAGGA GCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGATCAATGGGACCAAGTTCAG TTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGCTCTTTGCAGTGATCACGACCATCTTTTCG GCTGCTGAGAAGCAGTGGACCAATCTAGAGTGGAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACC ATTTTGGGCCGCATGGGTTAGTTTTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCG CTCCACATCTATAGTGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGA ATTCTAGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAAACGCA CGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTGCTGGGTTGGTGCATC GGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAAAACAGGCGAAATTCTTACAAGATGT ACCAGTCTTTCGGTGATGATGAATACAAGGACAAGGAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGG AGATAGGGCCTGCATTCATTGATAATGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAA TGACAGCACTGCAGATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCAC GTTAACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCATCATA TTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCAGTCCCTGACCACTGT CTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTCCAGCTTGAAGGTGGGTCTGAGGTA TTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGTTTCAGAGGGATTAGTGTGATACCCGCAGAATCGA GTGTCTAA SEQ ID NO 3: Cinnamomum camphora (Cc) FATB1b variant M25L, M322R, AT367-D368 coding DNA sequence codon optimized for Prototheca moriformis TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGAAACCCAGGA GCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGATCAATGGGACCAAGTTCAG TTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGCTCTTTGCAGTGATCACGACCATCTTTTCG GCTGCTGAGAAGCAGTGGACCAATCTAGAGTGGAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACC ATTTTGGGCCGCATGGGTTAGTTTTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCG CTCCACATCTATAGTGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGA ATTCTAGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAAACGCA CGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTGCTGGGTTGGTGCATC GGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAAAACAGGCGAAATTCTTACAAGATGT ACCAGTCTTTCGGTGATGATGAATACAAGGACAAGGAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGG AGATAGGGCCTGCATTCATTGATAATGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAA TGACAGCACTGCAGATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCAC GTTAACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCATCATA TTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCAGTCCCTGACCACTGT CTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTCCAGCTTGAAGGTGGGTCTGAGGTA TTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGTTTCAGAGGGATTAGTGTGATACCCGCAGAATCGA GTGTCTAA SEQ ID NO: 4 Cinnamomum camphora (Cc) FATB4 amino acid sequence MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEGLKGCSTLQGQSMLD DHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAARNHAESLGLLGDGFGETLEMSKRDLIWVVR RTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRDCKTGHILTRCTSVSVMMNMRTRRLSKIPQEVR AEIDPLFIEKVAVKEGEIKKLQKLNDSTADYIQGGWTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENH HLSSITLEYRRECTRGNKLQSLTTVCGGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERF PQQEPHK SEQ ID NO: 5 Cinnamomum camphora (Cc) FATB4 coding DNA sequence ATGGTCACCACCTCTTTAGCTTCCGCTTACTTCTCGATGAAAGCTGTAATGTTGGCTCCTGACGGCAGGG GCATAAAGCCCAGGAGCAGTGGTTTGCAGGTGAGGGCGGGAAATGAACGAAACTCTTGCAAGGTGATCAA TGGGACCAAGGTCAAAGACACGGAGGGCTTGAAAGGGTGCAGCACGTTGCAAGGCCAGAGCATGCTTGAT GACCATTTTGGTCTGCATGGGCTAGTTTTCAGGCGCACCTTTGCAATCAGATGCTATGAGGTTGGACCTG ACCGCTCCACATCCATAATGGCTGTTATGAATCACTTGCAGGAAGCTGCACGTAATCATGCGGAGAGTCT GGGACTTCTAGGAGATGGATTCGGTGAGACACTGGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAGA CGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGCGATACTGTTGAAGTCGAGGCCTGGGTGGGTG CATCAGGTAACACTGGCATGCGCCGCGATTTCCTTGTCCGCGACTGCAAAACTGGCCACATTCTTACAAG ATGTACCAGTGTTTCAGTGATGATGAATATGAGGACAAGGAGATTGTCCAAAATTCCCCAAGAAGTTAGA GCGGAGATTGACCCTCTTTTCATTGAAAAGGTTGCTGTCAAGGAAGGGGAAATTAAAAAATTACAGAAGT TGAATGATAGCACTGCAGATTACATTCAAGGGGGTTGGACTCCTCGATGGAATGATTTGGATGTCAATCA GCACGTGAACAATATCATATACGTTGGCTGGATTTTTAAGAGCGTCCCAGACTCTATCTCTGAGAATCAT CATCTTTCTAGCATCACTCTCGAATACAGGAGAGAGTGCACAAGGGGCAACAAGCTGCAGTCCCTGACCA CTGTTTGTGGTGGCTCGTCGGAAGCTGGGATCATATGTGAGCACCTACTCCAGCTTGAGGATGGGTCTGA GGTTTTGAGGGCAAGAACAGAGTGGAGGCCCAAGCACACCGATAGTTTCCAAGGCATTAGTGAGAGATTC CCGCAGCAAGAACCGCATAAGTAA SEQ ID NO: 6 Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGACCACCTCCCTGGCCTCCGCCTACTTCTCCATGAAGGCCGTGATGCTGGCCCCCGACGGCCGCG GCATCAAGCCCCGCTCCTCCGGCCTGCAGGTGCGCGCCGGCAACGAGCGCAACTCCTGCAAGGTGATCAA CGGCACCAAGGTGAAGGACACCGAGGGCCTGAAGGGCTGCTCCACCCTGCAGGGCCAGTCCATGCTGGAC GACCACTTCGGCCTGCACGGCCTGGTGTTCCGCCGCACCTTCGCCATCCGCTGCTACGAGGTGGGCCCCG ACCGCTCCACCTCCATCATGGCCGTGATGAACCACCTGCAGGAGGCCGCCCGCAACCACGCCGAGTCCCT GGGCCTGCTGGGCGACGGCTTCGGCGAGACCCTGGAGATGTCCAAGCGCGACCTGATCTGGGTGGTGCGC CGCACCCACGTGGCCGTGGAGCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGGAGGCCTGGGTGGGCG CCTCCGGCAACACCGGCATGCGCCGCGACTTCCTGGTGCGCGACTGCAAGACCGGCCACATCCTGACCCG CTGCACCTCCGTGTCCGTGATGATGAACATGCGCACCCGCCGCCTGTCCAAGATCCCCCAGGAGGTGCGC GCCGAGATCGACCCCCTGTTCATCGAGAAGGTGGCCGTGAAGGAGGGCGAGATCAAGAAGCTGCAGAAGC TGAACGACTCCACCGCCGACTACATCCAGGGCGGCTGGACCCCCCGCTGGAACGACCTGGACGTGAACCA GCACGTGAACAACATCATCTACGTGGGCTGGATCTTCAAGTCCGTGCCCGACTCCATCTCCGAGAACCAC CACCTGTCCTCCATCACCCTGGAGTACCGCCGCGAGTGCACCCGCGGCAACAAGCTGCAGTCCCTGACCA CCGTGTGCGGCGGCTCCTCCGAGGCCGGCATCATCTGCGAGCACCTGCTGCAGCTGGAGGACGGCTCCGA GGTGCTGCGCGCCCGCACCGAGTGGCGCCCCAAGCACACCGACTCCTTCCAGGGCATCTCCGAGCGCTTC CCCCAGCAGGAGCCCCACAAGTGA SEQ ID NO: 7 Cinnamomum camphora (Cc) FATB3 amino acid sequence MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHASPKINGSKVSTDTL KGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRPDMLADPFGIGRFMQDGLIFR QHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPA WGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDV AIIEEDNRKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRR ECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKSANNSRSILEMPAESL SEQ ID NO: 8 Cinnamomum camphora (Cc) FATB3 coding DNA sequence ATGGTTGCCACCGCTGCTGCTTCTGCTTTCTTCCCGGTCGGTGCTCCGGCTACGTCATCTGCAACTTCAG CCAAAGCGTCGATGATGCCTGATAATTTGGATGCCAGAGGCATCAAACCGAAGCCGGCTTCGTCCAGCGG CTTGCAGGTTAAGGCAAATGCCCATGCCTCTCCCAAGATTAATGGTTCCAAGGTGAGCACGGATACCTTG AAGGGGGAAGACACCTTAACTTCCTCGCCCGCCCCACGGACCTTTATCAACCAATTGCCTGACTGGAGCA TGTTCCTTGCTGCCATCACAACTATTTTCTTGGCTGCCGAGAAGCAGTGGACGAATCTCGACTGGAAGCC CAGAAGACCCGACATGCTTGCTGACCCGTTTGGCATCGGGAGGTTTATGCAGGATGGGCTGATTTTCAGG CAGCACTTTGCAATCAGATCTTATGAGATTGGGGCTGATAGAACGGCGTCTATAGAGACTTTAATGAATC ACTTGCAGGAGACTGCACTTAATCATGTGAGGAGTGCTGGACTCCTAGGTGATGGATTTGGTGCGACACC TGAGATGAGTAGAAGAGATCTGATATGGGTTGTAACACGTATGCAGGTTCTTGTGGACCGCTACCCTGCT TGGGGTGATATTGTTGAAGTAGAGACCTGGGTTGGTGCATCTGGAAAAAATGGTATGCGCCGTGATTGGC TTGTTCGGGACAGCCAAACTGGTGAAATTCTCACACGAGCTACCAGTGTTTGGGTGATGATGAATAAACG GACAAGGCGATTGTCCAAACTTCCTGAAGAAGTTAGAGGGGAAATAGGGCCTTATTTTATAGAAGATGTT GCTATCATAGAGGAGGACAACAGGAAACTACAGAAGCTCAATGAAAACACTGCTGATAATGTTCGAAGGG GTTTGACTCCTCGCTGGAGTGATCTGGATGTTAATCAGCATGTGAACAATGTCAAATACATTGGTTGGAT TCTTGAGAGTGCACCAGGATCCATCTTGGAGAGTCATGAGCTTTCCTGCATGACCCTTGAATACAGGAGA GAATGTGGGAAGGACAGTGTGCTGCAGTCAATGACTGCTGTCTCTGGTGGAGGCAGTGCAGCAGGTGGCT CACCAGAATCTAGCGTTGAGTGTGACCACTTGCTCCAGCTAGAGAGTGGGCCTGAAGTTGTGAGGGGAAG AACCGAGTGGAGGCCCAAGAGTGCTAATAACTCGAGGAGCATCCTGGAGATGCCGGCCGAGAGC SEQ ID NO: 9 Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCGCCTCCGCCTTCTTCCCCGTGGGCGCCCCCGCCACCTCCTCCGCCACCTCCG CCAAGGCCTCCATGATGCCCGACAACCTGGACGCCCGCGGCATCAAGCCCAAGCCCGCCTCCTCCTCCGC CCTGCAGGTGAAGGCCAACGCCCACGCCTCCCCCAAGATCAACGGCTCCAAGGTGTCCACCGACACCCTG AAGGGCGAGGACACCCTGACCTCCTCCCCCGCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCA TGTTCCTGGCCGCCATCACCACCATCTTCCTGGCCGCCGAGAAGCAGTGGACCAACCTGGACTGGAAGCC CCGCCGCCCCGACATGCTGGCCGACCCCTTCGGCATCGGCCGCTTCATGCAGGACGGCCTGATCTTCCGC CAGCACTTCGCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCGCCCTGAACCACGTGCGCTCCGCCGGCCTGCTGGGCGACGGCTTCGGCGCCACCCC CGAGATGTCCCGCCGCGACCTGATCTGGGTGGTGACCCGCATGCAGGTGCTGGTGGACCGCTACCCCGCC TGGGGCGACATCGTGGAGGTGGAGACCTGGGTGGGCGCCTCCGGCAAGAACGGCATGCGCCGCGACTGGC TGGTGCGCGACTCCCAGACCGGCGAGATCCTGACCCGCGCCACCTCCGTGTGGGTGATGATGAACAAGCG CACCCGCCGCCTGTCCAAGCTGCCCGAGGAGGTGCGCGGCGAGATCGGCCCCTACTTCATCGAGGACGTG GCCATCATCGAGGAGGACAACCGCAAGCTGCAGAAGCTGAACGAGAACACCGCCGACAACGTGCGCCGCG GCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCGCCCCCGGCTCCATCCTGGAGTCCCACGAGCTGTCCTGCATGACCCTGGAGTACCGCCGC GAGTGCGGCAAGGACTCCGTGCTGCAGTCCATGACCGCCGTGTCCGGCGGCGGCTCCGCCGCCGGCGGCT CCCCCGAGTCCTCCGTGGAGTGCGACCACCTGCTGCAGCTGGAGTCCGGCCCCGAGGTGGTGCGCGGCCG CACCGAGTGGCGCCCCAAGTCCGCCAACAACTCCCGCTCCATCCTGGAGATGCCCGCCGAGTCCCTGTGA SEQ ID NO: 10 Cuphea hyssopifolia (Chs) FATB1 amino acid sequence MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGALPLLASICKCPKK ADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAGYFGDSFGATPEMTKRNLIW VITKMQVLVDRYPAWGDVVQVDTWTCSSGKNSMQRDWFVRDLKTGDIITRASSVWVLMNRLTRKLSKIPE AVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYVLSGLTPRWSDLDMNQHVNNVKYIAWILESVPQSIPE THKLSAITVEYRRECGKNSVLQSLTNVSGDGITCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGA PIQAEKVYNNK* SEQ ID NO: 11 Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence ATGGTTGCCACTAATGCTGCTGCCTTTTCTGCTTATACTTTCTTCCTTACTTCACCAACTCATGGTTACT CTTCCAAACGTCTCGCCGATACTCAAAATGGTTATCCGGGTACCTCCTTGAAATCGAAATCCACTCCTCC ACCAGCTGCTGCTGCTGCTCGTAACGGTGCATTGCCACTGCTGGCCTCCATCTGCAAATGCCCCAAAAAG GCTGATGGGAGTATGCAACTAGACAGCTCCTTGGTCTTCGGGTTTCAATTTTACATTAGATCATATGAAG TGGGTGCGGATCAAACCGTGTCAATACAGACAGTACTCAATTACTTACAGGAGGCAGCCATCAATCATGT TCAGAGTGCTGGCTATTTTGGTGATAGTTTTGGCGCCACCCCGGAAATGACCAAGAGGAACCTCATCTGG GTTATCACTAAGATGCAGGTTTTGGTGGATCGCTATCCCGCTTGGGGCGATGTTGTTCAAGTTGATACAT GGACCTGTAGTTCTGGTAAAAACAGCATGCAGCGTGATTGGTTCGTACGGGATCTCAAAACTGGAGATAT TATAACAAGAGCCTCGAGCGTGTGGGTGCTGATGAATAGACTCACCAGAAAATTATCAAAAATTCCTGAA GCAGTTCTGGAAGAAGCAAAACTTTTTGTGATGAACACTGCCCCCACCGTAGATGACAACAGGAAGCTAC CAAAGCTGGATGGCAGCAGTGCTGATTATGTCCTCTCTGGCTTAACTCCTAGATGGAGCGACTTAGATAT GAACCAGCATGTCAACAATGTGAAGTACATAGCCTGGATCCTTGAGAGTGTCCCTCAGAGCATACCGGAG ACACACAAGCTGTCAGCGATAACCGTGGAGTACAGGAGAGAATGTGGCAAGAACAGCGTCCTCCAGTCTC TGACCAACGTCTCCGGGGATGGAATCACATGTGGAAACAGTATTATCGAGTGCCACCATTTGCTTCAACT TGAGACTGGCCCAGAGATTCTACTAGCGCGGACGGAGTGGATATCCAAGGAACCTGGGTTCAGGGGAGCT CCAATCCAGGCAGAGAAAGTCTACAACAACAAATAA SEQ ID NO: 12 Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCAACGCCGCCGCCTTCTCCGCCTACACCTTCTTCCTGACCTCCCCCACCCACGGCTACT CCTCCAAGCGCCTGGCCGACACCCAGAACGGCTACCCCGGCACCTCCCTGAAGTCCAAGTCCACCCCCCC CCCCGCCGCCGCCGCCGCCCGCAACGGCGCCCTGCCCCTGCTGGCCTCCATCTGCAAGTGCCCCAAGAAG GCCGACGGCTCCATGCAGCTGGACTCCTCCCTGGTGTTCGGCTTCCAGTTCTACATCCGCTCCTACGAGG TGGGCGCCGACCAGACCGTGTCCATCCAGACCGTGCTGAACTACCTGCAGGAGGCCGCCATCAACCACGT GCAGTCCGCCGGCTACTTCGGCGACTCCTTCGGCGCCACCCCCGAGATGACCAAGCGCAACCTGATCTGG GTGATCACCAAGATGCAGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACGTGGTGCAGGTGGACACCT GGACCTGCTCCTCCGGCAAGAACTCCATGCAGCGCGACTGGTTCGTGCGCGACCTGAAGACCGGCGACAT CATCACCCGCGCCTCCTCCGTGTGGGTGCTGATGAACCGCCTGACCCGCAAGCTGTCCAAGATCCCCGAG GCCGTGCTGGAGGAGGCCAACCTGTTCGTGATGAACACCGCCCCCACCGTGGACGACAACCGCAAGCTGC CCAAGCTGGACGGCTCCTCCGCCGACTACGTGCTGTCCGGCCTGACCCCCCGCTGGTCCGACCTGGACAT GAACCAGCACGTGAACAACGTGAAGTACATCGCCTGGATCCTGGAGTCCGTGCCCCAGTCCATCCCCGAG ACCCACAAGCTGTCCGCCATCACCGTGGAGTACCGCCGCGAGTGCGGCAAGAACTCCGTGCTGCAGTCCC TGACCAACGTGTCCGGCGACGGCATCACCTGCGGCAACTCCATCATCGAGTGCCACCACCTGCTGCAGCT GGAGACCGGCCCCGAGATCCTGCTGGCCCGCACCGAGTGGATCTCCAAGGAGCCCGGCTTCCGCGGCGCC CCCATCCAGGCCGAGAAGGTGTACAACAACAAGTGA SEQ ID NO: 13 Cuphea hyssopifolia (Chs) FATB2 amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVGLKSGSLK TQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAP VIEDDDRKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS* SEQ ID NO: 14 Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTAGACCTGGAA AGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGGCCAATGGCGGGTTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTTCGGTCGGTCTAAAGTCCGGCAGTCTCAAG ACTCAGGAAGACACTCCTTCGGCGCCTCCTCCCCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAACCACT TGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTTGGTCGTACTCTTGA GATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGAC AAGAAGATTGTCAAAAATTCCAGATGAGGTTCGACATGAGATAGAGCCTCATTTCGTGGACTCTGCTCCC GTCATTGAAGATGATGACCGGAAACTTCCCAAGCTGGATGAGAAGACTGCTGACTCCATCCGCAAGGGTC TAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCT TGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATATAGGCGGGAA TGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTCTGGGTCTCAGT TCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATTGTGAAGGGGAGAACTGAGTGGCGACCCAAGAC TGCAGGAATCAATGGGCCAATAGCATCCGGGGAGACCTCACCTGGAGACTCTTCTTAG SEQ ID NO: 15 Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCCAACGGCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCTCCCTGAAG ACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTTCGGCCGCACCCTGGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCC TGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTCCGGCTCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAC CGCCGGCATCAACGGCCCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTCCTCCTGA SEQ ID NO: 16 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVGLKSGSLK TQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSKSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVR HEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQ ELCSLTLEYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGE TSPGDSS* SEQ ID NO: 17 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant coding DNA sequence ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTAGACCTGGAA AGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGGCCAATGGCGGGTTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTTCGGTCGGTCTAAAGTCCGGCAGTCTCAAG ACTCAGGAAGACACTCCTTCGGCGCCTCCTCCdCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAACCACT TGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTTGGTCGTACTCTTGA GATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACTAGAGCATCAAGTAAAAGCCAAATTATGTTACCCTTACA TTATTGCAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGA CATGAGATAGAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAAGATGATGACCGGAAACTTCCCAAGC TGGATGAGAAGACTGCTGACTCCATCCGCAAGGGTCTAACTCCGAAGTGGAATGACTTGGATGTCAATCA GCACGTCAACAACGTGAAGTACATTGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAG GAGTTATGTTCCCTTACCCTGGAATATAGGCGGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTG CTGTGGACCCCTCTGGAAAGGGCTCTGGGTCTCAGTTCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGA GATTGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGAATCAATGGGCCAATAGCATCCGGGGAG ACCTCACCTGGAGACTCTTCTTAG SEQ ID NO: 18 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCCAACGGCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCTCCCTGAAG ACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTTCGGCCGCACCCTGGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCAAGTCCCAGATCATGCTGCCCCTGCA CTACTGCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGC CACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACGACCGCAAGCTGCCCAAGC TGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCA GCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAG GAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG CCGTGGACCCCTCCGGCAAGGGCTCCGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGA GATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAACGGCCCCATCGCCTCCGGCGAG ACCTCCCCCGGCGACTCCTCCTGA SEQ ID NO: 19 Cuphea hyssopifolia (Chs) FATB3 amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSAVSLKSGSL DTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLMDPFGVDRVVQDGAVF RQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYP TWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDS APVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRR ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 20 Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTAAACCCGGGA AGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAAACCACAATGGCGGCTTTCA GGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTC GACACTCAGGAGGACACTTCATCGTCGTCCTCTCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGA GTATGCTGCTGTCCGCGATCACGACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAA ATCTAAGAGGCCCGACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTC AGACAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGA ACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTAC TCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAATGCACGTCGAGGTTAATCGCTATCCT ACTTGGGGTGATACTATCGAGGTCAATACTTGGGTCTCCGAGTCGGGGAAAACCGGTATGGGTCGTGATT GGCTGATAAGTGATTGTCATACAGGAGAAATTCTAATAAGAGCAACGAGCATGTGTGCTATGATGAATCA AAAGACGAGAAGATTCTCAAAATTTCCATATGAGGTTCGACAGGAGTTGGCGCCTCATTTTGTGGACTCT GCTCCTGTCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCAATG GCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGAT TCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTGGCCTCACCCTTGAGTATAGGCGG GAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATCCATCAAAAGAGGGAGACAGATCTC TGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAA GAATGCAGGAACCAATGGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 21 Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAACCACAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCCCTG GACACCCAGGAGGACACCTCCTCCTCCTCCTCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGT CCATGCTGCTGTCCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTC CGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGCACGTGGAGGTGAACCGCTACCCC ACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACT GGCTGATCTCCGACTGCCACACCGGCGAGATCCTGATCCGCGCCACCTCCATGTGCGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCC GCCCCCGTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGCAACG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCC TGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 22 Cuphea hyssopifolia (Chs) FATB3b (V204I, C239F, E243D, M251V variant) amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSAVSLKSGSL DTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLMDPFGVDRVVQDGAVF RQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYP TWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDS APVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRR ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 23 Cuphea hyssopifolia (Chs) FATB3b (V204I, C239F, E243D, M251V variant) coding DNA sequence ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTAAACCCGGGA AGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAAACCACAATGGCGGCTTTCA GGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTC GACACTCAGGAGGACACTTCATCGTCGTCCTCTCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGA GTATGCTGCTGTCCGCGATCACGACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAA ATCTAAGAGGCCCGACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTC AGACAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGA ACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTAC TCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAATGCACATCGAGGTTAATCGCTATCCT ACTTGGGGTGATACTATCGAGGTCAATACTTGGGTCTCCGAGTCGGGGAAAACCGGTATGGGTCGTGATT GGCTGATAAGTGATTTTCATACAGGAGACATTCTAATAAGAGCAACGAGCGTGTGTGCTATGATGAATCA AAAGACGAGAAGATTCTCAAAATTTCCATATGAGGTTCGACAGGAGTTAGCGCCTCATTTTGTGGACTCT GCTCCAGTCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCAATG GCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGAT TCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTGGCCTCACCCTTGAGTATAGGCGG GAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATCCCTCAAAAGAGGGAGACAGATCTC TGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAA GAATGCAGGAACCAATGGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 24 Cuphea hyssopifolia (Chs) FATB3b (V204I, C239F, E243D, M251V variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAACCACAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCCCTG GACACCCAGGAGGACACCTCCTCCTCCTCCTCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGT CCATGCTGCTGTCCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTC CGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGCACATCGAGGTGAACCGCTACCCC ACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACT GGCTGATCTCCGACTTCCACACCGGCGACATCCTGATCCGCGCCACCTCCGTGTGCGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCC GCCCCCGTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGCAACG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCC TGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 25 Cuphea PSR23 (Cu) FATB3 amino acid sequence MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKANGSAVNLKSGSL NTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKCIVRDGLVSR QSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTKMQIMVNRYPT WGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSP HVIEDNDQKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS* SEQ ID NO: 26 Cuphea PSR23 (Cu) FATB3 coding DNA sequence ATGGTGGTGGCTGCAGCAACTTCTGCATTCTTCCCCGTTCCAGCCCCGGGAACCTCCCCTAAACCCGGGA AGTCCGGCAACTGGCCATCGAGCTTGAGCCCTACCTTCAAGCCCAAGTCAATCCCCAATGCCGGATTTCA GGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTGGCAGCCTC AACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCCCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTA TGCTTCTGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATAGGAAATC TAAGAGGCCTGACATGCTCGTGGACTCGGTTGGGTTGAAGTGTATTGTTCGGGATGGGCTCGTGTCCAGA CAGAGTTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGAACC ACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCTTTGGTCGTACTCC TGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTACCCAACT TGGGGCGATACTGTTGAGATCAATACCTGGTTCTCTCAGTCGGGGAAAATCGGTATGGCTAGCGATTGGC TAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCAAAA GACGAGAAGATTCTCAAGACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCT CATGTCATTGAAGACAATGATCAGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCAAGG GTCTAACTCCGAGGTGGAACGACTTGGATGTGAATCAGCACGTAAGCAACGTGAAGTACATTGGGTGGAT TCTCGAGAGTATGCCAATAGAAGTTTTGGAGACACAGGAGCTATGCTCTCTCACCGTAGAATATAGGCGG GAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCTGTGGATCCCTCAGAAAATGGAGGCCGGTCTC AGTACAAGCACCTTCTGCGGCTTGAGGATGGGACTGATATCGTGAAGAGCAGAACTGAGTGGCGACCGAA GAATGCAGGAACTAACGGGGCGATATCAACATCAACAGCAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 27 Cuphea PSR23 (Cu) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCbCCAAGCCCGGCA AGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCATCCCCAACGCCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG AACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCA TGCTGCTGACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTC CAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCCTGAAGTGCATCGTGCGCGACGGCCTGGTGTCCCGC CAGTCCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGGCTTCGGCCGCACCCC CGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCACC TGGGGCGACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGCCTCCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAA GACCCGCCGCTTCTCCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCC CACGTGATCGAGGACAACGACCAGAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCGTGGAGTACCGCCGC GAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACCGCCGTGGACCCCTCCGAGAACGGCGGCCGCTCCC AGTACAAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 28 Cuphea wrightii (Cw) FATB3 amino acid sequence MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPKANGSAVSLKSGS LNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPDMLVDWFGSETIVQDGLVF RERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYP TWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYR RECGRESVVESVTSMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST* SEQ ID NO: 29 Cuphea wrightii (Cw) FATB3 coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTAGAACCACGCCTAAACCCG GGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCGCCCTTCAAGCCCAAGTCAAACCCCAATGGTAGATT TCAGGTTAAGGCAAATGTCAGTCCTCATCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCTGGAGGACCCTCCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTCACTAGGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTAGTGGACTGGTTTGGGTCAGAGACTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGATTTTtGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGA ACCACTTGCAGGACACATCTCTGAATCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CTCGGAGATGTGTACAAGAGACCTCATTTGGGTGCTTACAAAAATGCAGATCGTGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCGGGGAAAATCGGTATGGGTCGCGATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTGTAAGAGCAACGAGCGCTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCATGCGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCT CCTCCTGTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCTACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGTGGAAGGGAAAGTGTGGTAGAGTCCGTGACCTCTATGAATCCCTCAAAAGTTGGAGACCGGT CTCAGTACCAACACCTTCTGCGGCTTGAGGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGAGACC AAAGAATGCAGGAACCAACCGGGCGATATCAACATGA SEQ ID NO: 30 Cuphea wrightii (Cw) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCCGCACCACCCCCAAGCCCG GCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCCCCTTCAAGCCCAAGTCCAACCCCAACGGCCGCTT CCAGGTGAAGGCCAACGTGTCCCCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCTGGAGGACCCCCCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTTCACCCGCCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCTCCGAGACCATCGTGCAGGACGGCCTGGTGTTC CGCGAGCGCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CTCCGAGATGTGCACCCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCGTGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCGACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGGTGCGCGCCACCTCCGCCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCTGCGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGAGTCCGTGGTGGAGTCCGTGACCTCCATGAACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACCGCGCCATCTCCACCTGA SEQ ID NO: 31 Cuphea wrightii (Cw) FATB4a amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGLKSGGF KTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGSIVQDGLVFR QNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPT WGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSA PVVEDDDRKLPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRR ECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGDFF* SEQ ID NO: 32 Cuphea wrightii (Cw) FATB4a coding DNA sequence TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTGCCATCCGCCGACACCTCCTCCTCGAGACCCG GAAAGCTCGGCAGTGGACCATCGAGCTTGAGCCCCCTCAAGCCCAAATCGATCCCCAATGGCGGCTTGCA GGTTAAGGCAAACGCCAGTG6CCCTCCTAAGATCAATGGTTCCTCGGTCGGTCTAAAGTCGGGCGGTTTC AAGACTCAGGAAGACTCTCCTTCGGCCCCTCCTCCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGTA TGCTTCTTGCTGCAATCACTACTGTCTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACC TAAGAGGCCTGACATGCTCGTGGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTGTTCAGG CAGAATTTTTCAATTAGGTCCTACGAAATAGGCGCCGATCGAACTGCGTCTATAGAGACGGTGATGAACC ATTTGCAGGAAACAGCTCTCAATCATGTCAAGATTGCTGGGCTTTCTAATGACGGCTTTGGTCGTACTCC TGAGATGTATAAAAGAGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCATGGTTAACCGCTATCCTACT TGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGACTGGC TCATAAGTGATTGCAATACTGGAGAGATTCTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAA GACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCT CCCGTCGTTGAAGATGATGATCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAGG GTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATCGGATGGAT TCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGCTCCCTGACCCTGGAATACAGGCGG GAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTCGACCCGTCTGCAGAGGGCTATGCGTCCC GGTTTCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCCAA GAATGCTGGAATCAATGGGGTGGTACCATCCGAGGAGTCCTCACCTGGAGACTTCTTTTAG SEQ ID NO: 33 Cuphea wrightii (Cw) FATB4a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTCCCGCCCCG GCAAGCTGGGCTCCGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATCCCCAACGGCGGCCTGCA GGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCGGCTTC AAGACCCAGGAGGACTCCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCA TGCTGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCC CAAGCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGC CAGAACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACC ACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCAACGACGGCTTCGGCCGCACCCC CGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACC TGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAA GACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCC CCCGTGGTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCACCCOCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGCCGAGGGCTACGCCTCCC GCTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCATCAACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTTCTTCTGA SEQ ID NO: 34 Cuphea wrightii (Cw) FATB4b amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGLKSGSF KTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGSIVQDGLVFR QNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGRTPAMSKRDLIWVVAKMQVMVNRYPA WGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSA PVVEDDDRKLPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRR ECGRESVLESLTAVDPSGEGDGSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGGDFF* SEQ ID NO: 35 Cuphea wrightii (Cw) FATB4b coding DNA sequence TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTACCATCCGCCGACACCTCCTCATCGAGACCCG GAAAGCTCGGCAATGGGCCATCGAGCTTGAGCCCCCTCAAGCCGAAATCGATCCCCAATGGCGGGTTGCA GGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCTCGGTCGGTCTGAAGTCGGGCAGTTTC AAGACTCAGGAAGACGCTCCTTCGGCCCCTCCTCCTCGGACTTTTATCAACCAGTTGCCTGATTGGAGTA TGCTTCTTGCTGCAATCACTACTGTCTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACC TAAGAGGCCTGACATGCTTGTCGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTTTTCAGG CAGAATTTCTCGATTAGGTCCTACGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAACC ATTTGCAGGAAACAGCTCTCAATCATGTTAAGATTGCTGGGCTTTCTAGTGATGGCTTTGGTCGTACTCC TGCGATGTCTAAACGGGACCTCATTTGGGTTGTTGCGAAAATGCAGGTCATGGTTAACCGCTACCCTGCT TGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGACTGGC TCATAAGTGATTGCAACACTGGAGAGATTCTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAA GACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCG CCCGTCGTTGAAGACGATGACCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAGG GTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGAT TCTTGAGAGTACTCCAGCAGAAGTTCTGGAGACCCAGGAATTATGTTCCCTGACCCTGGAATACAGGCGG GAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTAGATCCGTCTGGAGAGGGCGATGGGTCCA AGTTCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCAAA GAATGCTGGAATCAATGGGGTGGTACCATCCGAGGAGTCCTCACCTGGTGGAGACTTCTTTTAA SEQ ID NO: 36 Cuphea wrightii (Cw) FATB4b coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTCCCGCCCCG GCAAGCTGGGCAACGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATCCCCAACGGCGGCCTGCA GGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCTCCTTC AAGACCCAGGAGGACGCCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCA TGCTGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCC CAAGCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGC CAGAACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACC ACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCTCCGACGGCTTCGGCCGCACCCC CGCCATGTCCAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCGCC TGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAA GACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCC CCCGTGGTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCACCCCCGCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCGAGGGCGACGGCTCCA AGTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCATCAACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGGCGACTTCTTCTGA SEQ ID NO: 37 Cuphea wrightii (Cw) FATB5 amino acid sequence MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANGSAVNLKSGSLETP PRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGVDRTASI ETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNG MGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGD SIRDGLTPRWNDLDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKE GDRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVVRLIF* SEQ ID NO: 38 Cuphea wrightii (Cw) FATB5 coding DNA sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCTGTTCCAACCCCGGGAACGCCCCCTAAACCCGGGA AGTTCGGTAACTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGACAATGGTGGCTTTCATGTCAAGGC AAACGCCAGTGCCCATCCTAAGGCTAATGGTTCTGCGGTAAATCTAAAGTCTGGCAGCCTCGAGACTCCT CCTCGGAGTTTCATTAACCAGCTGCCGGACTTGAGTGTGCTTCTGTCCAAAATCACGACTGTCTTCGGGG CGGCTGAGAAGCAGTGGAAGAGGCCCGGCATGCTCGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGA TGGTGTTTtTTTCAGACAGAGTTTTTCTATCAGGTCTTACGAAATAGGCGTTGATCGAACAGCCTCGATA GAGACACTGATGAACATCTTCCAGGAAACATCTTTGAATCATTGCAAGAGTATCGGTCTTCTCAACGATG GCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGT GAATCGCTATCCTACTTGGGGTGACACTATCGAAGTCAATACTTGGGTCTCGGAGTCGGGGAAAAACGGT ATGGGTCGGGATTGGCTGATAAGTGATTGCCGTACTGGAGAGATTCTTATAAGAGCAACGAGCGTGTGGG CGATGATGAATCAAAACACGAGAAGATTGTCAAAATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCA TTTTGTGGACTCTGCTCCTGTCATTGAAGACGATCAAAAGTTGCAGAAGCTTGATGTGAAGACAGGTGAT TCCATTCGCGATGGTCTAACTCCGAGATGGAATGACTTGGATGTCAATCAACACGTTAACAATGTGAAGT ACATTGGATGGATTCTCAAGAGTGTTCCAATAGAAGTTTTCGAGACACAGGAGCTATGCGGCGTCACACT TGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCAGTGACCGCTATGGATCCAGCAAAAGAG GGAGACCGGTGTGTGTACCAGCACCTTCTTCGGCTTGAGGATGGAGCTGATATCACTATAGGCAGAACCG AGTGGCGGCCGAAGAATGCAGGAGCCAATGGTGCAATGTCATCAGGAAAGACTTCAAATGGAAACTGTCT CATAGAAGGAAGGGGTTGGCAACCTTTCCGAGTTGTGCGTTTAATTTTCTGA SEQ ID NO: 39 Cuphea wrightii (Cw) FATB5 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCCCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGACAACGGCGGCTTCCACGTGAAGGC CAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCCC CCCCGCTCCTTCATCAACCAGCTGCCCGACCTGTCCGTGCTGCTGTCCAAGATCACCACCGTGTTCGGCG CCGCCGAGAAGCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGA CGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGTGGACCGCACCGCCTCCATC GAGACCCTGATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACG GCTTCGGCCQCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGT GAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGGC ATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGUGAGATCCTGATCCGCGCCACCTCCGTGTGGG CCATGATGAACCAGAACACCCGCCGCCTGTCCAAGTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCA CTTCGTGGACTCCGCCCCCGTGATCGAGGACGACCAGAAGCTGCAGAAGCTGGACGTGAAGACCGGCGAC TCCATCCGCGACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGT ACATCGGCTGGATCCTGAAGTCCGTGCCCATCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCT GGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCGCCAAGGAG GGCGACCGCTGCGTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCATCGGCCGCACCG AGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATGTCCTCCGGCAAGACCTCCAACGGCAACTGCCT GATCGAGGGCCGCGGCTGGCAGCCCTTCCGCGTGGTGCGCCTGATCTTCTGA SEQ ID NO: 40 Cuphea heterophylla (Cht) FATB1a amino acid sequence MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPKANGSAVNLKSGSL ETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEPFGVDRIFQDGVFFRQSFSIR SYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIE VNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDD KKLHKLDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSV LESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 41 Cuphea heterophylla (Cht) FATB1a coding DNA sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCCCGGGAACCTCCACTAAACCCGGGA ACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAAACCACAATGGTGGCTTTCG GGTCAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTGGCAGCCTC GAGACTCAGGAGGACACTTCATCGTCGTCCCCTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGG GTATGCTTCTGTCCAAAATCACGACTGTCTTCGGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCT TGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGG TCTTACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAAACATCTC TGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGA CCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCTATCCTACTTGGGGTGATACTATTGAG GTCAATACTTGGGTCTCAGAGTCGGGGAAAAACGGTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTA CCGGAGAAATTCTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAA ATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGAT AAAAAATTGCACAAGCTTGATGTTAAGACGGGTGATTCCATTCGCAAGGGTCTAACTCCAAGGTGGAATG ACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAAGAGTGTTCCAGCAGA AGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTACAGGCGGGAATGTGGAAGGGACAGTGTG CTGGAGTCCGTGACCGCTATGGATACCGCAAAAGAGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGC TTGAGGATGGGGCTGATATCACCATAGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGC AATATCAACAGGAAAGACTTCAAATGAAAACTCTGTCTCTTAG SEQ ID NO: 42 Cuphea heterophylla (Cht) FATB1a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCTCCACCAAGCCCGGCA ACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCAACCACAACGGCGGCTTCCG CGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG GAGACCCAGGAGGACACCTCCTCCTCCTCCCCCCCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGG GCATGCTGCTGTCCAAGATCACCACCGTGTTCGGCGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCT GGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGC TCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGACCTCCC TGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGA CCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAG GTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCGCA CCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAA GTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGAC AAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAAGTCCGTGCCCGCCGA GGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTG CTGGAGTCCGTGACCGCCATGGACACCGCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCC TGGAGGACGGCGCCGACATCACCATCGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGC CATCTCCACCGGCAAGACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 43 Cuphea heterophylla (Cht) FATB1b (P16S, T20P, G94S, G105W, S293F, L305F variant) amino acid sequence MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPESSHNGGFQVKANASAHPKANGSAVNLKSGSL ETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRPGMLVEPFGVDRIFQDGVFFRQSFSIR SYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIE VNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDD KKLHKLDVKTGDFIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSV LESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 44 Cuphea heterophylla (Cht) FATB1b (P16S, T2OP, G94S, G105W, S293F, L305F variant) coding DNA sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCTCGGGAACCTCCCCTAAACCCGGGA ACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAAGCCACAATGGTGGCTTTCA GGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTGGCAGCCTC GAGACTCAGGAGGACACTTCATCGTCGTCCCCTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGA GTATGCTTCTGTCCAAAATCACGACTGTCTTCTGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCT TGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGG TCTTACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAAACATCTC TGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGA CCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCTATCCTACTTGGGGTGATACTATTGAG GTCAATACTTGGGTCTCAGAGTCGGGGAAAAACGGTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTA CCGGAGAAATTCTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAA ATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGAT AAAAAATTGCACAAGCTTGATGTTAAGACGGGTGATTTCATTCGCAAGGGTCTAACTCCAAGGTGGAATG ACTTTGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAAGAGTGTTCCAGCAGA AGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTATAGGCGGGAATGTGGAAGGGACAGTGTG CTGGAGTCCGTGACCGCTATGGATACCGCAAAAGAGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGC TTGAGGATGGGGCTGATATCACCATAGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGC AATATCAACAGGAAAGACTTCAAATGAAAACTCTGTCTCTTAG SEQ ID NO: 45 Cuphea heterophylla (Cht) FATB1b (P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCTCCGGCACCTCCCCCAAGCCCGGCA ACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCTCCCACAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG GAGACCCAGGAGGACACCTCCTCCTCCTCCCCCCCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGT CCATGCTGCTGTCCAAGATCACCACCGTGTTCTGGGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCT GGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGC TCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGACCTCCC TGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGA CCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAG GTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCGCA CCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAA GTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGAC AAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTTCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAAGTCCGTGCCCGCCGA GGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTG CTGGAGTCCGTGACCGCCATGGACACCGCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCC TGGAGGACGGCGCCGACATCACCATCGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGC CATCTCCACCGGCAAGACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 46 Cuphea heterophylla (Cht) FATB2b amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVDWFGLEIIVQDGLVF RESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 47 Cuphea heterophylla (Cht) FATB2b coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCCGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGGCACTTCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA GTCTAAGAAGCCTGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGATAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 48 Cuphea heterophylla (Cht) FATB2b coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGGCACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGAAGCCCGACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 49 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGSAVSLKSGS LNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVDWFGLEIIVQDWLVF RESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPLIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 50 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGTCTAAACCCG GGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTCAAACCCCAATGGTGGATT TCAGGTTAAGGCAAATGCCAGCGCTCATCCTAAGGCTAACGGGTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTAAGGAGGACACTCCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA GTCTAAGAAGCCTGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATTGGCTCGTGTTC AGAGAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTLGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGCCGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCTCCTCATTTTGTGGACGCC CCTCCTCTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 51 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCTCCAAGCCCG GCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTCCAACCCCAACGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCAAGGAGGACACCCCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGAAGCCCGACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACTGGCTGGTGTTC CGCGAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCCTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 52 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVDWFGLEIIVQDGLVF RESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 53 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCCGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTAAGGAGGACACTCCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA ATAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA GTCTAAGAAGCCTGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGGGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 54 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCAAGGAGGACACCCCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGA ACCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGAAGCCCGACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 55 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGSAVSLKSGS LNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDLFGLESIVQDGLVF RESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYR RECGRESVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 56 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGTCTAAACCCG GGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTCAAACCCCAATGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCGGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACTGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTCGTGGACTTGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTATTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAATACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCT CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCA AGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGAAAGTGTGCTGGAGTCCGTGACCGCTATGAATCCCTCAAAAGTTGGAGACCGGT CTCAGTACCAGCACCTTCTACGGCTTGAGGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 57 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCTCCAAGCCCG GCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTCCAACCCCAACGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACCTGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTACTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACtGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAACACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGCA AGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCGTGACCGCCATGAACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 58 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDWFGLESIVQDGLVF RESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYP NWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYR RECGRDSVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 59 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCAGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACAGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAAAGGCCTGACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGGTGATGA ACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCTTGGTGAATCGCTATCCA AATTGGGGTGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAATACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCT CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCA AGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGAATCCCTCAAAAGTTGGAGACCGGT CTCAGTACCAGCACCTTCTACGGCTTGAGGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 60 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCAGACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGTGATGA ACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCCTGGTGAACCGCTACCCC AACTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAACACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGCA AGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGAACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 61 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDWFGLESIVQDGLVF RESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYP NWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 62 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAAGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCCGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGGCACTTCGTCGTCCCCTCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACTGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGGTGATGA ACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCTTGGTGAATCGCTATCCA AATTGGGGTGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAATGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAACGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 63 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGGCACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGTGATGA ACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCCTGGTGAACCGCTACCCC AACTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 64 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S, A157T, H165L, T211N, G414A variant) amino acid sequence MVVAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDWFGLESIVQDGLVF REIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYP NWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS* SEQ ID NO: 65 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S, A157T, H165L, T211N, G414A variant) coding DNA sequence ATGGTGGTGGCTGCTACAGCAAGTTCTGCATTCTTCCCTGTTCCTGTACCTGGAACCTCCCCTAAACCCG GAAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACTGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGATTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAACCTCTATAGAGACGGTGATGA ACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCTTGGTGAATCGCTATCCA AATTGGGGTGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGCAAACTCGGTCTCTTAG SEQ ID NO: 66 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S, A157T, H165L, T211N, G414A variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCACCGCCTCCTCCGCCTTCTTCCCCGTGCCCGTGCCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGATCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCACCTCCATCGAGACCGTGATGA ACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCCTGGTGAACCGCTACCCC AACTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGACTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGCCAACTCCGTGTCCTGA SEQ ID NO: 67 Cuphea heterophylla (Cht) FATB3aamino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSLKSCSLK THEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAP VIEDDDWKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS* SEQ ID NO: 68 Cuphea heterophylla (Cht) FATB3a coding DNA sequence ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCGTCCCCGGACACCTCCTCTAGACCGGGAA AGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTGCCAATGCTGGGCTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCTCGGTCAGTCTAAAGTCTTGCAGTCTCAAG ACTCATGAAGACACTCCTTCAGCTCCTCCTCCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCCATAGAGACGGTGATGAACCACT TGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTAATGAAGGCTTTGGTCGTACTCCTGA GATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACAAGGGCATCAAGTGTGTGGGTCATGATGAATCAAAAGAC AAGAAAATTGTCAAAGATTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCC GTCATTGAAGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGGGTC TAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCT TGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATACAGGCGGGAA TGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTTTGGGCCCCAGT TTCAGCACCTTCTGAGGCTTGAGGATGGAGGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGAC TGCAGGTATCAATGCCACGATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG SEQ ID NO: 69 Cuphea heterophylla (Cht) FATB3a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCCAACGCCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGTCCCTGAAGTCCTGCTCCCTGAAG ACCCACGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGAGGGCTTCGGCCGCACCCCCGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCAAGCTGTCCAAGATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCC TGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAC CGCCGGCATCAACGGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 70 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N1791 variant) amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSLKSGSLK TQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGFGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAP VIEDDDWKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS* SEQ ID NO: 71 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCATCCCCGGACACCTCCTCTAGACCGGGAA AGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTGCCAATGCTGGGCTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCTCGGTCAGTCTAAAGTCTGGCAGTCTCAAG ACTCAGGAAGACACTCCTTCGGCTCCTCCTCCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTTGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCTATAGAGACGGTGATGAACCACT TGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTATTGAAGGCTTTGGTCGTACTCCTGA GATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGAC AAGAAAATTGTCAAAGATTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCC GTCATTGAAGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGGGTC TAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCT TGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATACAGGCGGGAA TGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTTTGGGCCCCAGT TTCAGCACCTTCTGAGGCTTGAGGATGGAGGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGAC TGCAGGTATCAATGGGACGATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG SEQ ID NO: 72 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCCAACGCCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGTCCCTGAAGTCCGGCTCCCTGAAG ACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCTTCGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGATCGAGGGCTTCGGCCGCACCCCCGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCAAGCTGTCCAAGATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCC TGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAC CGCCGGCATCAACGGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 73 Cuphea viscosissima (Cvis) FATB1 amino acid sequence MVAAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNGGFQVKANASAHPKANGSAVNLKSGSL NTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKSIVRDGLVSR HSFSIRSYEIGADRTASIETLMNHLQETTINHCKSLGLHNDGFGRTPGMCKNDLIWVLTKMQIMVNRYPT WGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSP HVIEDNDQKLRKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS SEQ ID NO: 74 Cuphea viscosissima (Cvis) FATB1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGG6CGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCAAGCCCGGCA AGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCATCCCCAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG AACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCA TGCTGCTGACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTC CAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCCTGAAGTCCATCGTGCGCGACGGCCTGGTGTCCCGC CACTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCACCATCAACCACTGCAAGTCCCTGGGCCTGCACAACGACGGCTTCGGCCGCACCCC CGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCACC TGGGGCGACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGCCTCCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAA GACCCGCCGCTTCTCCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCC CACGTGATCGAGGACAACGACCAGAAGCTGCGCAAGTTCGACGTGAAGACCGGCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCGTGGAGTACCGCCGC GAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACCGCCGTGGACCCCTCCGAGAACGGCGGCCGCTCCC AGTACAAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 75 Cuphea viscosissima (Cvis) FATB2 amino acid sequence MVATAASSAFFPVPSADTSSRPGKLGNGPSSFSPLKPKSIPNGGLQVKASASAPPKINGSSVGLKSGGLK THDDAPSAPPPRTFINQLPDWSMLLAAITTAFLAAEKQWMMLDRKPKRLDMLEDPFGLGRVVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKTAGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRREIEPHFVDSAP VIEDDDRKLPKLDEKSADSIRKGLTPRWNDLDVNQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGEGYGSQFQHLLRLEDGGEIVKGRTEWRPKNAGINGVVPSEESSPGDYS SEQ ID NO: 76 Cuphea viscosissima (Cvis) FATB2 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCCCCTCCTCCTTCTCCCCCCTGAAGCCCAAGTCCATCCCCAACGGCGGCCTGCAGGT GAAGGCCTCCGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCGGCCTGAAG ACCCACGACGACGCCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGCCTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACCGCAAGCCCAA GCGCCTGGACATGCTGGAGGACCCCTTCGGCCTGGGCCGCGTGGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTAAGACCGCCGGCCTGTCCAACGACGGCTTCGGCCGCACCCCCGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCAAGCTGTCCAAGATCCCCGACGAGGTGCGCCGCGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGTCCGCCGACTCCATCCGCAAGGGCC TGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGCCAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCGAGGGCTACGGCTCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAA CGCCGGCATCAACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTACTCCTGA SEQ ID NO: 77 Cuphea viscosissima (Cvis) FATB3 amino acid sequence MVAAAASSAFFSFPTPGTSPKPGKFGNWPSSLSIPFNPKSNHNGGIQVKANASAHPKANGSAVSLKAGSL ETQEDTSSPSPPPRTFISQLPDWSMLVSAITTVFVAAEKQWTMLDRKSKRPDVLVEPFVQDGVSFRQSFS IRSYEIGVDRTASIETLMNIFQETSLNHCKSLGLLNDGFGRTPEMCKRDLIWVVTKMQIEVNRYPTWGDT IEVTTWVSESGKNGMSRDWLISDCHSGEILIRATSVWAMMNQKTRRLSKIPDEVRQEIVPYFVDSAPVIE DDRKLHKLDVKTGDSIRNGLTPRWNDFDVNQHVNNVKYIAWLLKSVPTEVFETQELCGLTLEYRRECRRD SVLESVTAMDPSKEGDRSLYQHLLRLENGADIALGRTEWRPKNAGATGAVSTGKTSNGNSVS SEQ ID NO: 78 Cuphea viscosissima (Cvis) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCTTCCCCACCCCCGGCACCTCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCTCCTCCCTGTCCATCCCCTTCAACCCCAAGTCCAACCACAACGGCGGCATCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGGCCGGCTCCCTG GAGACCCAGGAGGACACCTCCTCCCCCTCCCCCCCCCCCCGCACCTTCATCTCCCAGCTGCCCGACTGGT CCATGCTGGTGTCCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACGTGCTGGTGGAGCCCTTCGTGCAGGACGGCGTGTCCTTCCGCCAGTCCTTCTCC ATCCGCTCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGA CCTCCCTGAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGAGATGTGCAA GCGCGACCTGATCTGGGTGGTGACCAAGATGCAGATCGAGGTGAACCGCTACCCCACCTGGGGCGACACC ATCGAGGTGACCACCTGGGTGTCCGAGTCCGGCAAGAACGGCATGTCCCGCGACTGGCTGATCTCCGACT GCCACTCCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCCT GTCCAAGATCCCCGACGAGGTGCGCCAGGAGATCGTGCCCTACTTCGTGGACTCCGCCCCCGTGATCGAG GACGACCGCAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAACGGCCTGACCCCCCGCT GGAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGCCTGGCTGCTGAAGTCCGTGCC CACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAGTACCGCCGCGAGTGCCGCCGCGAC TCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGC TGCGCCTGGAGAACGGCGCCGACATCGCCCTGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAC CGGCGCCGTGTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA 

1. An isolated nucleic acid having at least 80% sequence identity to any of SEQ ID NOS: 21, 27, 30, 69, 72, or 78 or any equivalent sequences by virtue of the degeneracy of the genetic code.
 2. An isolated nucleic acid sequence encoding a protein having at least 80% sequence identity to any of SEQ ID NO: 22, 25, 28, 67, 70, or 77, or a fragment thereof having acyl-ACP thioesterase activity.
 3. The isolated nucleic acid of claim 2, wherein, the protein has acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell comprising that sequence.
 4. A method of producing a recombinant cell that produces an altered fatty acid profile, the method comprising transforming the cell with a nucleic acid according to claim
 1. 5. A host cell produced by the method of claim
 4. 6. The host cell of claim 5, wherein the host cell is selected from a plant cell, a microbial cell, and a microalgal cell.
 7. A method for producing an oil or oil-derived product, the method comprising cultivating a host cell of claim 5, and extracting oil produced thereby, optionally wherein the cultivation is heterotrophic growth on sugar.
 8. The method of claim 7, further comprising producing a fatty acid, fuel, chemical, or other oil-derived product from the oil.
 9. An oil produced by the method of claim 7, optionally having a fatty acid profile comprising at least 20% C14 or C16 fatty acids.
 10. An oil-derived product produced by the method of claim
 8. 11. The oil of claim 10, wherein the oil is produced by a microalgae and optionally, lacks C24-alpha sterols. 